文献类型：期刊文献

英文题名：SSR GENOTYPING-GENETIC DIVERSITY AND FINGERPRINTING OF TEAK (TECTONA GRANDIS) CLONES

作者：Huang, G. H.[] Liang, K. N.[1] Zhou, Z. Z.[] Ma, H. M.[]

第一作者：黄桂华

通信作者：Liang, KN[1]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Guangdong, Peoples R China; Chinese Acad Forestry, Natl Key Lab Forest Tree Genet & Breeding, Guangzhou 510520, Guangdong, Peoples R China

年份：2016

卷号：28

期号：1

起止页码：48-58

外文期刊名：JOURNAL OF TROPICAL FOREST SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000369517800006)】；

基金：This work was financially supported by the Fundamental Research Funds for Central Nonprofit Research Institution of RITF-CAF (No. RITFYWZX2011-06) and National Natural Science Foundation of China (No. 31200501) and also supported by the National 12th Five-Year Forestry Programme (No. 2012BAD01B0504). The authors would like to thank XM Wei from Sugar Research Australia for help in English. Thanks are also due to JM Xu, SM Gan and CL Zhong from the Chinese Academy of Forestry for help and advice.

语种：英文

外文关键词：Clone discrimination; clone characterisation; microsatellite marker; genetic variation; genetic relationship; clone management

摘要：A DNA-based fingerprint technique was developed for teak with 15 microsatellite markers, based on 26 widely cultivated teak clones. The same set of markers was used to investigate genetic variations and relationships of the 26 teak clones. Using the fingerprint technique, all teak clones could be unequivocally identified. In terms of genetic diversity among 26 clones, 160 alleles were detected with mean of 10.67 alleles per locus. The observed heterozygosity ranged from 0.3333 to 1.0000, averaging 0.6567. Despite the limited sample, there was substantial genetic variation that could be exploited in teak breeding programme. In addition, the cluster analysis separated teak clones of India origin from Myanmar and essentially confirmed historically known or speculated origin of the clones. Implication on clonal identification and management as well as the protection of legitimate interests of breeders and growers was discussed.