文献类型：期刊文献

英文题名：Characterization of the Populus Rab family genes and the function of PtRabE1b in salt tolerance

作者：Zhang, Jin[1,2] Li, Yu[1] Liu, Bobin[1] Wang, Lijuan[1] Zhang, Li[1] Hu, Jianjun[1] Chen, Jun[1] Zheng, Huanquan[3] Lu, Mengzhu[1]

第一作者：Zhang, Jin

通信作者：Wang, LJ[1];Lu, MZ[1]|[a0005e27922ec495458cc]王丽娟;

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat State Forestry A, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[2]Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN USA;[3]McGill Univ, Biol Dept, Dev Biol Res Initiat, Montreal, PQ, Canada

年份：2018

卷号：18

期号：1

外文期刊名：BMC PLANT BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000436117900004)】；

基金：This work was supported by the National Natural Science Foundation of China [31270699] to J.C. and a Collaborative Innovation Plan of Jiangsu Higher Education to M.L. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

外文关键词：Populus; Rab GTPase; Phylogenetic analysis; Co-expression network; Subcellular localization; Salt tolerance

摘要：Background: Rab proteins form the largest family of the Ras superfamily of small GTP-binding proteins and regulate intracellular trafficking pathways. However, the function of the Rab proteins in woody species is still an open question. Results: Here, a total of 67 PtRabs were identified in Populus trichocarpa and categorized into eight subfamilies (RabA-RabH). Based on their chromosomal distribution and duplication blocks in the Populus genome, a total of 27 PtRab paralogous pairs were identified and all of them were generated by whole-genome duplication events. Combined the expression correlation and duplication date, the PtRab paralogous pairs that still keeping highly similar expression patterns were generated around the latest large-scale duplication (similar to 13 MYA). The cis-elements and co-expression network of unique expanded PtRabs suggest their potential roles in poplar development and environmental responses. Subcellular localization of PtRabs from each subfamily indicates each subfamily shows a localization pattern similar to what is revealed in Arabidopsis but RabC shows a localization different from their counterparts. Furthermore, we characterized PtRabE1b by overexpressing its constitutively active mutant PtRabE1b(Q74L) in poplar and found that PtRabE1b(Q74L) enhanced the salt tolerance. Conclusions: These findings provide new insights into the functional divergence of PtRabs and resources for genetic engineering resistant breeding in tree species.