文献类型：期刊文献

英文题名：Differences in phyllosphere microbiomes among different Populus spp. in the same habitat

作者：Liu, Jiaying[1,2] Zhang, Weixi[2,3] Liu, Yuting[1] Zhu, Wenxu[1,2,4] Yuan, Zhengsai[2,3] Su, Xiaohua[2,3] Ding, Changjun[2,3]

第一作者：Liu, Jiaying

通信作者：Ding, CJ[1];Ding, CJ[2]

机构：[1]Shenyang Agr Univ, Coll Forestry, Shenyang, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat State Forestry Ad, Beijing, Peoples R China;[4]Shenyang Agr Univ, Coll Forestry, Res Stn Liaohe River Plain Forest Ecosyst, Chinese Forest Ecosyst Res Network CFERN, Tieling, Peoples R China

年份：2023

卷号：14

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000967747900001)】；

基金：This work was supported by the National Key Research and Development Program of China (grant number 2021YFD2201205), and the Basic Research Fund of CAF (grant number CAFYBB2020SZ002).

语种：英文

外文关键词：phyllosphere microorganism; microbiomes; Populus spp; phyllosphere microbial community; phyllosphere

摘要：IntroductionThe above-ground parts of terrestrial plants are collectively known as the phyllosphere. The surface of the leaf blade is a unique and extensive habitat for microbial communities. Phyllosphere bacteria are the second most closely associated microbial group with plants after fungi and viruses, and are the most abundant, occupying a dominant position in the phyllosphere microbial community. Host species are a major factor influencing the community diversity and structure of phyllosphere microorganisms. MethodsIn this study, six Populus spp. were selected for study under the same site conditions and their phyllosphere bacterial community DNA fragments were paired-end sequenced using 16S ribosomal RNA (rRNA) gene amplicon sequencing. Based on the distribution of the amplicon sequence variants (ASVs), we assessed the alpha-diversity level of each sample and further measured the differences in species abundance composition among the samples, and predicted the metabolic function of the community based on the gene sequencing results. ResultsThe results revealed that different Populus spp. under the same stand conditions resulted in different phyllosphere bacterial communities. The bacterial community structure was mainly affected by the carbon and soluble sugar content of the leaves, and the leaf nitrogen, phosphorus and carbon/nitrogen were the main factors affecting the relative abundance of phyllosphere bacteria. DiscussionPrevious studies have shown that a large proportion of the variation in the composition of phyllosphere microbial communities was explained by the hosts themselves. In contrast, leaf-borne nutrients were an available resource for bacteria living on the leaf surface, thus influencing the community structure of phyllosphere bacteria. These were similar to the conclusions obtained in this study. This study provides theoretical support for the study of the composition and structure of phyllosphere bacterial communities in woody plants and the factors influencing them.