文献类型：期刊文献

中文题名：相思根瘤菌培养基优化及其固氮酶活性研究

英文题名：Study of Medium Optimization of Acacia Rhizobium and Nitrogenase Activity

作者：尚军红[1] 康丽华[1] 罗玉萍[2] 江业根[1] 马海宾[1]

第一作者：尚军红

机构：[1]中国林业科学研究院热带林业研究所;[2]南昌大学

年份：2005

卷号：15

期号：1

起止页码：72-74

中文期刊名：生物技术

外文期刊名：Biotechnology

收录：CSTPCD;;CSCD:【CSCD_E2011_2012】；

基金：国家"十五"攻关项目 (2 0 0 2BA51 5B0 2 );国家"863"计划项目 (2 0 0 3AA2 4 1 1 533)中部分内容

语种：中文

中文关键词：相思根瘤菌(MZ、YM2);培养基优化;正交实验;固氮酶活性;乙炔还原法

外文关键词：Allorhizobium Acacia(MZ,YM2);Medium Optimization;Orthogonal test;Nitrogenase Activity;Acetylene-reduction method

分类号：S791.248

摘要：研究了 2株相思根瘤菌在 4种培养基中的生长情况 ,并在此基础上应用正交实验L9(34 )对相思根瘤菌进行优化培养基研究 ,筛选出相思根瘤菌优化培养基配方。结果表明 :MZ菌株与YM2菌株在各自优化培养基的菌体数量高达 8.2× 10 9个 mL与 6 .3× 10 9个 mL ,分别为原有培养基的 3.6 4倍与 1.14倍 ;另外用乙炔还原法测得它们的最高固氮酶活性分别为 0 .96 3μg .mL- 1 .d- 1 与 0 .2 80 μg .mL- 1 .d- 1 。
The growth behavior of Acacia Rhizobium(MZ and YM2) in four mediums were studied in this paper.Accordingly,their optimized mediums were obtained from L_9(34)orthogonal test.The results showed that the number of MZ and YM2 were respectively 8.2×109/mL and 6.3×109/mL in their own optimized medium,which were 3.64 and 1.14 times of their conventional mediums.The study of nitrogenase activity by Acetylene reduction method showed that MZ and YM2 had comparatively high nitrogen-fixation activity,which reached 0.963μg.mL(-1).d(-1) and 0.280 μg.mL(-1).d(-1).