文献类型：期刊文献

英文题名：De novo Characterization of the Platycladus orientalis Transcriptome and Analysis of Photosynthesis-Related Genes during Aging

作者：Chang, Ermei[1] Zhang, Jin[1,2] Yao, Xiamei[3] Tang, Shuo[4] Zhao, Xiulian[1] Deng, Nan[1] Shi, Shengqing[1] Liu, Jianfeng[1] Jiang, Zeping[1,5]

第一作者：常二梅

通信作者：Jiang, ZP[1];Jiang, ZP[2]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat, Natl Forestry & Grassland Adm,Res Inst Forestry, Beijing 100091, Peoples R China;[2]Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN 37831 USA;[3]Fuyang Normal Univ, Fuyang 236037, Peoples R China;[4]Zhongshan Pk, Beijing 100031, Peoples R China;[5]Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, Beijing 100091, Peoples R China

年份：2019

卷号：10

期号：5

外文期刊名：FORESTS

收录：;EI(收录号：20192407034566);Scopus(收录号：2-s2.0-85066851136);WOS:【SCI-EXPANDED(收录号:WOS:000478814700032)】；

基金：This work was supported by the National Non-Profit Research Institutions of the Chinese Academy of Forestry [grant numbers: CAFYBB2017ZA007] and the National Natural Science Foundation of China [grant number 31870664].

语种：英文

外文关键词：longevity; Platycladus orientalis; senescence; transcriptome; gene expression

摘要：In China, Platycladus orientalis has a lifespan of thousands of years. The long lifespan of these trees may be relevant for the characterization of plant aging at the molecular level. However, the molecular mechanism of the aging process of P. orientalis is still unknown. To explore the relationship between age and growth of P. orientalis, we analyzed physiological changes during P. orientalis senescence. The malondialdehyde content was greater in 200-, 700-, and 1100-year-old ancient trees than in 20-year-old trees, whereas the peroxidase and superoxide dismutase activities, as well as the soluble protein content, exhibited the opposite trend. Furthermore, we performed a de novo transcriptome assembly using RNA-Seq and obtained 48,044 unigenes with an average length of 896 bp. A total of 418 differentially expressed genes were identified in different stages of aging of P. orientalis. Clustering analysis revealed distinct timepoints at which the oxidation-reduction and photosynthesis pathways changed. Eight clusters with distinct expression patterns were identified. The expression levels of photosynthesis-, oxidation-reduction-, and transporter-related genes were down-regulated, whereas those of transcription-, signaling-, and senescence-related genes were up-regulated during aging. In addition, consistent with the most obviously down-regulated genes of photosynthesis-related genes, the photosynthetic indexes including chlorophyll a and b levels decreased steadily during P. orientalis aging. This study combined transcriptome with physiological and biochemical data, revealing potential candidate genes influencing senescence during P. orientalis aging.