文献类型：期刊文献

中文题名：青麸杨腺毛结构、化学组分及基因表达解析

英文题名：The Histological Structure,Chemical Constituents and Gene Expression of Glandular Trichomes of Rhus potaninii

作者：陆沁[1,2] 王超[3] 邵淑霞[1] 刘娟[1] 陈航[1]

第一作者：陆沁

机构：[1]中国林业科学研究院高原林业研究所,云南昆明650224;[2]南京林业大学林学院,江苏南京210037;[3]西南林业大学园林学院,云南昆明650233

年份：2023

卷号：43

期号：2

起止页码：54-61

中文期刊名：西南林业大学学报：自然科学

外文期刊名：Journal of Southwest Forestry University:Natural Sciences

收录：CSTPCD;;北大核心:【北大核心2020】；

基金：中国林业科学研究院面上项目(CAFYBB2021SY012)资助;云南省基础研究计划青年项目(2019FD027)资助;云南省教育厅科学研究基金项目(2021J0172)资助。

语种：中文

中文关键词：腺毛;组织结构;WRKY33;PTI6;基因表达

外文关键词：glandular trichome;histological structure;WRKY33;PTI6;gene expression

分类号：S792

摘要：通过石蜡切片、组织染色及实时荧光定量技术研究青麸杨腺毛,旨在探究青麸杨腺毛的组织结构、化学组分及基因调控机理。结果表明:青麸杨腺毛呈松塔状,有1个由(12.00±4.14)个分泌细胞构成的头部及无细胞结构的柄部构成,在排出分泌物后,腺毛逐渐萎缩并凋落。腺毛的分泌物中含有大量的粘性多糖、酚类物质及酸性脂质,这些分泌物在为幼嫩的叶片提供物理保护层,防止水分丧失的同时,还可有效防止病原微生物在幼嫩叶片表面大量繁殖。通过转录组分析结合实时荧光定量PCR验证,鉴定出在腺毛中特异表达的候选关键基因,其中,与植物—病原菌互作相关的基因WRKY33和PTI6,及参与调控对昆虫有毒副作用的氰基氨基酸代谢的基因SLC36A在腺毛中的表达量较叶片中均显著上调。
The aim of this study was to investigate the structure,chemical composition and gene regulation mechanism of glandular trichome(GT)on Rhus potaninii.The GTs was studied by paraffin section,tissue staining and real-time fluorescence quantification.We found the GTs look like pinecone and consist by a head with 12.00±4.14 cells and an acellular stalk.The GTs became thinner after secreting and fall down at later stage.The secretion is composed of lots of polysaccharides,phenolic compounds,and acidic lipids,it could protect the young leaves from drought and pathogen in the surface.Furthermore,we found the selectively expressed genes in the GTs based on transcriptome and identified it by quantitative real-time polymerase chain reaction(RT-qPCR).The WRKY33 and PTI6 which regulated the plant-pathogen interaction were enhanced in the GTs,meanwhile,SLC36A which regulated the Cyanoamino acid metabolism(Cyanoamino acid has toxic or side effects to insects)was enhanced too.