文献类型：期刊文献

英文题名：A facultative ectomycorrhizal association is triggered by organic nitrogen

作者：Peng, Long[1,2] Zhang, Yan[3] Druzhinina, Irina S.[4] Kubicek, Christian P.[5] Wang, Yuchen[1,2] Zhu, Zhiyong[2] Zhang, Yuwei[1,2] Wang, Kexuan[1,2] Liu, Zhuo[1,2] Zhang, Xiaoguo[2] Martin, Francis[6] Yuan, Zhilin[1,2]

第一作者：彭龙

通信作者：Yuan, ZL[1];Yuan, ZL[2];Martin, F[3]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Dongxiaofu 1, Beijing, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Daqiao Rd 73, Hangzhou 311400, Peoples R China;[3]Liaoning Prov Inst Poplar, Gaizhou 115213, Peoples R China;[4]Royal Bot Gardens, Richmond, England;[5]TU Wien, Inst Chem Environm & Biosci Engn ICEBE, Vienna, Austria;[6]Univ Lorraine, Ctr INRAE Grand Est Nancy, INRAE, UMR 1136 Interact Arbres Microorganismes, F-54280 Champenoux, France

年份：2022

卷号：32

期号：24

起止页码：5235-+

外文期刊名：CURRENT BIOLOGY

收录：;Scopus(收录号：2-s2.0-85144040039);WOS:【SCI-EXPANDED(收录号:WOS:000909650600001)】；

基金：This work was funded by Fundamental Research Funds for the Central Non-profit Research of Chinese Academy of Forestry (CAFYBB2020QB002), National Key Research and Development Program of China (2022YFD2201900), and National Natural Science Foundation of China (no. 31901290 and 31722014). Research in FMM laboratory is funded by the Laboratory of Excellence Advanced Research on the Biology of Tree and Forest Ecosystems (ARBRE; grant ANR-11-LABX-0002-01). We are grateful to Prof. Guanzheng Qu (Northeast Forestry University, China) for providing the poplar cells.

语种：英文

外文关键词：Clitopilus hobsonii; ectomycorrhizae; nitrogen mineralization; plant cell-wall degrading enzymes; Populus; saprotrophy

摘要：Increasing nitrogen (N) deposition often tends to negatively impact the functions of belowground ectomycorrhizal networks, although the exact molecular mechanisms underlying this trait are still unclear. Here, we assess how the root-associated fungus Clitopilus hobsonii establishes an ectomycorrhiza-like association with its host tree Populus tomentosa and how this interaction is favored by organic N over mineral N. The establishment of a functional symbiosis in the presence of organic N promotes plant growth and the transfer of 15N from the fungus to above ground plant tissues. Genomic traits and in planta transcriptional signatures suggest that C. hobsonii may have a dual lifestyle with saprotrophic and mutualistic traits. For example, several genes involved in the digestion of cellulose and hemicellulose are highly expressed during the interaction, whereas the expression of multiple copies of pectin-digesting genes is tightly controlled. Conversely, the nutritional mutualism is dampened in the presence of ammonium (NH4+) or nitrate (NO3-). Increasing levels of NH4+ led to a higher expression of pectin-digesting genes and a continuous increase in hydrogen peroxide production in roots, whereas the presence of NO3- resulted in toxin production. In summary, our results suggest that C. hobsonii is a facultative ectomycorrhizal fungus. Access to various forms of N acts as an on/off switch for mutualism caused by large-scale fungal physiological remodeling. Furthermore, the abundance of pectin-degrading enzymes with distinct expression patterns during functional divergence after exposure to NH4+ or organic N is likely to be central to the transition from parasitism to mutualism.