文献类型：期刊文献

中文题名：四点象天牛线粒体全基因组结构及其在天牛科的系统发育地位

英文题名：Complete Mitochondrial Genome Structure of Mesosa myops and its Phylogenetic Status in Cerambycidae

作者：渠子琪[1] 李承锦[1] 李长帅[2] 崔研[2] 杨明亮[2] 张苏芳[1] 刘福[1] 孔祥波[1] 方加兴[1]

第一作者：渠子琪

机构：[1]中国林业科学研究院森林生态环境与自然保护研究所,国家林业和草原局森林保护学重点实验室,北京100091;[2]北京百花山国家级自然保护区,北京102311

年份：2025

卷号：5

期号：1

起止页码：54-64

中文期刊名：陆地生态系统与保护学报

外文期刊名：Terrestrial Ecosystem and Conservation

基金：北京百花山国家级自然保护区生物多样性调查项目(91133-2023)。

语种：中文

中文关键词：天牛科;四点象天牛;线粒体基因组;系统发育分析

外文关键词：Cerambycidae;Mesosa myops;mitochondrial genome;phylogenetic analysis

分类号：Q963

摘要：【目的】四点象天牛(Mesosa myops)是主要的林业蛀干害虫,为了明确四点象天牛线粒体基因组结构特征和该物种在天牛科昆虫中的系统发育地位,进一步认识天牛科(Cerambycidae)的系统发育以及提升四点象天牛的分子鉴定、种群监测水平提供数据支持。【方法】采用Illumina HiSeq 4000平台对四点象天牛线粒体全基因组进行测序,并对其进行拼接、注释和分析。利用线粒体基因组中的13个蛋白编码基因通过贝叶斯法(Bayesian inference,BI)和最大似然法(maximum likelihood,ML)分析四点象天牛在天牛科中的系统进化地位。【结果】四点象天牛线粒体基因组全长为16130 bp,由37个基因组成,包括13个蛋白质编码基因(protein coding genes,PCGs)、2个核糖体RNA基因(rRNA)和22个转运RNA基因(tRNA),以及1个非编码控制区(control region,CR)。四点象天牛的线粒体基因组的碱基组成表现为明显的AT偏向性,AT碱基的含量为77.03%。13个PCGs均以标准的ATN为起始密码子,除nad5以单独T结尾外,其余PCGs均以传统的TAN为终止密码子,每种氨基酸使用频率最高的密码子为NNA和NNU,最频繁使用的氨基酸由高到低依次为Leu、Ile和Phe。22个tRNA基因中,除trnS1缺失DHU臂外,其余tRNA基因均为典型的三叶草形。基于GenBank中天牛科30个物种的线粒体基因组的13PCGs的蛋白序列,构建了具有较高支持率的四点象天牛的系统发育树,确认了该物种属于天牛科昆虫中的沟胫天牛亚科,四点象天牛与拟象天牛属的桑拟象天牛聚为一支,具有较近的亲缘关系。【结论】解析了四点象天牛线粒体基因组的结构特征,明确了四点象天牛在天牛科物种中的系统发育地位,丰富了天牛科的线粒体基因组数据库,也为该物种的分子鉴定及防治提供了新的数据支持。
【Objective】Mesosa myops is a major forestry trunk-boring pest.To clarify the structural characteristics of the mitochondrial genome of M.myops and the phylogenetic position of this species in the Cerambycidae of insects,this study aims to enrich the understanding of the phylogeny of Cerambycidae,as well as provide data support for the improvement of the molecular identification and population monitoring of M.myops.【Method】The complete mitochondrial genome of M.myops was sequenced on the Illumina HiSeq 4000 sequencing platform and then spliced,annotated and analyzed.Bayesian inference(BI)and maximum likelihood(ML)were used to re-establish the evolutionary relationships between M.myops and other insects in the Cerambycidae through 13 protein-coding genes.【Result】The complete mitochondrial genome of M.myops is 16130 bp in full-length,which contains 37 genes including 13 protein-coding genes(PCGs),2 rRNA genes,22 tRNA genes,and a control region(CR).The base composition exhibits a strong AT bias,with an A+T content of 77.03%.Except for nad5,all the 13 PCGs initiate with the standard start codon of ATN,which has an incomplete stop codon(T-),the remaining PCGs terminate with the conventional stop codon TAN.The most frequently used codons for each amino acid are NNA and NNU,and the most frequently used amino acids in descending order were Leu,Ile and Phe.Among the 22 tRNA genes,except for trnS1 which lacks the DHU arm,the remaining tRNA genes have a typical cloverleaf structure.Based on the mitochondrial genome data of 30 species from Cerambycidae family from GenBank,the phylogenetic relationships between M.myops and other species within the Cerambycidae family were reconstructed,indicating that M.myops belongs to the Lamiinae with high bootstrap support,and M.myops is closely related to the Agelasta perplexa of the Agelasta genus.【Conclusion】This study analyzed the structural characteristics of the mitochondrial genome of M.myops,and clarified the phylogenetic status of this pest in the Cerambycidae,which enriched the mitochondrial genome database of the Cerambycidae,and also provided new data support for the molecular identification and control of this pest.