文献类型：期刊文献

英文题名：Genetic Diversity and Association Analysis among Germplasms of Diospyros kaki in Zhejiang Province Based on SSR Markers

作者：Xu, Yang[1] Cheng, Wenqiang[1] Xiong, Chunyan[1] Jiang, Xibing[1] Wu, Kaiyun[1] Gong, Bangchu[1]

第一作者：徐阳

通信作者：Gong, BC[1]|[a00054c9262859c460f14]龚榜初;

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China

年份：2021

卷号：12

期号：4

外文期刊名：FORESTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000643012800001)】；

基金：The study was financially supported by the Fundamental Research Funds of CAF (CAFYB B2017ZA004-3 and CAFYBB2017SY015) and the Key Agricultural New Varieties Breeding Projects (New Varieties of Persimmon and Jujube Breeding Projects) funded by the Zhejiang Province Science and Technology Department.

语种：英文

外文关键词：Diospyros kaki; microsatellite markers; genetic diversity; population structure; marker trait association

摘要：In subtropical to temperate regions, persimmon (Diospyros kaki Thunb.) is an economically important fruit crop cultivated for its edible fruits. Persimmons are distributed abundantly and widely in Zhejiang Province, representing a valuable resource for the breeding of new cultivars and studying the origin and evolution of persimmon. In this study, we elucidated the genetic structures and diversity patterns of 179 persimmon germplasms from 16 different ecologic populations in Zhejiang Province based on the analysis of 17 SSR markers. The results show that there was a medium degree of genetic diversity for persimmon found in Zhejiang Province. With the exception of the Tiantai Mountain and Xin'an River populations, we found extensive gene exchange had occurred among the other populations. The 179 D. kaki germplasms from the 16 populations could be separated into three distinct clusters (I, II, and III) with a higher mean pairwise genetic differentiation index (FST) (0.2714). Nearly all samples of Cluster-I were distributed inland. Cluster-II and Cluster-III contained samples that were widely distributed throughout Zhejiang Province including all samples from the coastal populations and the Northeast Plain populations. In addition, we performed association mapping with nine traits (fruit crude fiber content, fruit calcium content, fruit water content, fruit longitudinal diameter, fruit aspect ratio, seed width, seed length, leaf aspect ratio, and number of lateral veins) using these markers. This led to the identification of 13 significant marker-trait associations (MTAs; p < 0.00044, 0.1/228) using a general linear model, of which, six MTAs with a correlation coefficient (R-2) >10% were consistently represented in the general linear model with p < 0.00044 in the two models. The genetic structures and diversity patterns of the persimmon germplasms revealed in this study will provide a reference for the efficient conservation and further utilization of persimmon germplasms. The MTAs identified in this study will be useful for future marker-assisted breeding of persimmon.