文献类型：期刊文献

英文题名：Mesorhizobium acaciae sp nov., isolated from root nodules of Acacia melanoxylon R. Br.

作者：Zhu, Ya Jie[1] Lu, Jun Kun[1] Chen, Ying Long[2,3,4,5] Wang, Sheng Kun[1] Sui, Xin Hua[6] Kang, Li Hua[1]

通信作者：Kang, LH[1]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Guangdong, Peoples R China;[2]Chinese Acad Sci, Inst Soil & Water Conservat, Yangling 712100, Peoples R China;[3]Northwest A&F Univ, Yangling 712100, Peoples R China;[4]Univ Western Australia, Sch Earth & Environm, Perth, WA 6009, Australia;[5]Univ Western Australia, UWA Inst Agr, Perth, WA 6009, Australia;[6]China Agr Univ, Coll Biol Sci, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China

年份：2015

卷号：65

期号：10

起止页码：3558-3563

外文期刊名：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000366152100052)】；

基金：We thank Dr Yanming Jin and students of China Agricultural University for their valuable suggestions and experimental assistance. This study was supported by the Special Fund for Forest Scientific Research in the Public Welfare, China (Grant no. 201004075) and the Fundamental Research Funds for the Central Non-profit Research Institution of RITE (Grant no. RITFYWZX201207).

语种：英文

摘要：Three novel strains, RITF741(T), RITF1220 and RITF909, isolated from root nodules of Acacia melanoxylon in Guangdong Province of China, have been previously identified as members of the genus Mesorhizobium, displaying the same 16S rRNA gene RFLP pattern. Phylogenetic analysis of 16S rRNA gene sequences indicated that the three strains belong to the genus Mesorhizobium and had highest similarity (100.0 %) to Mesorhizobium plurifarium LMG 11892(T). Phylogenetic analyses of housekeeping genes recA, atpD and glnII revealed that these strains represented a distinct evolutionary lineage within the genus Mesorhizobium. Strain RITF741(T) showed >73 % DNA-DNA relatedness with strains RITF1220 and RITF909, but <60 % DNA-DNA relatedness with the closest type strains of recognized species of the genus Mesorhizobium. They differed from each other and from their closest phylogenetic neighbours by presence/absence of several fatty acids, or by large differences in the relative amounts of particular fatty acids. While showing distinctive features, they were generally able to utilize a wide range of substrates as sole carbon sources based on API 50CH and API 20NE tests. The three strains were able to form nodules with the original host Acacia melanoxylon and other woody legumes such as Acacia aneura, Albizia falcataria and Leucaena leucocephala. In conclusion, these strains represent a novel species belonging to the genus Mesorhizobium based on the data obtained in the present and previous studies, for which the name Mesorhizobium acaciae sp. nov. is proposed. The type strain is RITF741(T) (=CCBAU 101000(T)=JCM 30534(T)), the DNA G+C content of which is 64.1 mol% (T-m).