文献类型：期刊文献

中文题名：不同产地金枣柿遗传变异研究

英文题名：Genetic Variation of Jinzaoshi Persimmon from Different Regions in Zhejiang Province

作者：熊春艳[1] 龚榜初[1] 应尚蛟[2] 吴开云[1] 江锡兵[1] 赵献民[1,3]

第一作者：熊春艳

机构：[1]中国林业科学研究院亚热带林业研究所;[2]浙江省永康市林业局;[3]浙江省海宁市林业果树技术服务站

年份：2014

卷号：27

期号：2

起止页码：225-232

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：浙江省农业新品种选育重大科技专项项目(2012C12904-10)

语种：中文

中文关键词：金枣柿;表型性状;SSR;遗传变异

外文关键词：Jinzaoshi persimmon; phenotypic character： SSR; genetic diversity

分类号：S718.46

摘要：以浙江省内37份金枣柿和3份农家柿品种为材料,利用SSR分子标记技术,结合表型性状和果实营养成分指标,分析不同产地金枣柿遗传多样性。结果表明:金枣柿枝、叶、芽等表型性状具有丰富的形态多样性,14个表型性状的变异系数为7.23%23.54%,其中萼片长与柿蒂长变异系数最大,果形指数、叶形指数变异系数最小;果实营养成分在单株间的变异丰富,变异系数为9.58%66.21%,平均变异系数为37.89%,变异程度高于表型性状;21对SSR多态性引物可从40份材料中扩增出381个DNA位点,平均多态性位点百分率为92.89%,UPGMA聚类分析显示,所选SSR标记能将供试材料完全区分开,在遗传相似系数0.74时将40个试验单株分为3类,主坐标分析所得结果与聚类分析基本一致。该研究结果为金枣柿种质资源的科学利用提供了依据。
The germplasm resources of Jinzaoshi persimmon in Zhejiang Province were investigated,so as to probe the genetic diversity of Jinzaoshi persimmon from different regions.The genetic variation of collected germplams,including 37 Jinzaoshi and 3 local varieties,was analyzed by SSR molecular marker,together with phenotypic traits and fruit nutrients contents.The results showed Jinzaoshi persimmon possessed a rich diversity on morphology.The variable coefficient about 14 phenotypic characters ranged from 7.23％ to 23.54％.The variable coefficient of sepal length and persimmon calyx length were the largest.The fruit shape index and leaf index had the least variation.The fruit nutrient contents ranged from 9.58％ （soluble solid content） to 66.21％ （tannin content）.Twenty-one primers were used to amplify 381 SSR local in 40 test materials,with an average polymorphism rate of 92.89％.The cluster analysis conducted by UPGMA showed that the materials tested could be distinguished and divided into three major groups at the genetic similarity coefficient of 0.74,which was similar to the relationship of PCoA analysis.