文献类型：期刊文献

英文题名：Retrieving complete plastid genomes of endangered Guibourtia timber using hybridization capture for forensic identification and phylogenetic analysis

作者：Lin, Chuanyang[1,2] Lu, Yang[2,3] Liu, Shoujia[2,3] Wang, Zhaoshan[4] Yao, Lihong[1,5] Yin, Yafang[2,3] Jiao, Lichao[2,3]

第一作者：Lin, Chuanyang

通信作者：Yao, LH[1];Jiao, LC[2]

机构：[1]Inner Mongolia Agr Univ, Coll Mat Sci & Art Design, Hohhot 010018, Peoples R China;[2]Chinese Acad Forestry, Res Inst Wood Ind, Beijing 100091, Peoples R China;[3]Chinese Acad Forestry, Wood Collect, Beijing 100091, Peoples R China;[4]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China;[5]Northwest Univ, Collaborat Res Ctr Archaeol Silk Rd, Sch Culture Heritage, Sch Culture Heritage,China Cent Asia Belt & Rd Joi, Xian 710127, Peoples R China

年份：2024

卷号：69

外文期刊名：FORENSIC SCIENCE INTERNATIONAL-GENETICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001152957000001)】；

基金：This study was supported financially by the Fundamental Research Funds of Chinese Academy of Forestry (Grant CAFYBB2021QB003) and Open Project of China -Central Asia "the Belt and Road" Joint Laboratory on Human and Environment Research, Key Laboratory of Cultural Heritage Research and Conservation, Collaborative Research Centre for Archaeology of the Silk Roads, Northwest University (Grant 363022201019). We would like to express our gratitude to Dr. Xia Liu of Chongqing University of Arts and Sciences for her academic suggestions, and Mr. Di Xiao for his help on sample collection.

语种：英文

外文关键词：Guibourtia; Heartwood DNA; Hybridization capture; Origin tracking; Species identification

摘要：The high economic value and increased demand for timber have led to illegal logging and overexploitation, threatening wild populations. In this context, there is an urgent need to develop effective and accurate forensic tools for identifying endangered Guibourtia timber species to protect forest ecosystem resources and regulate their trade. In this study, a hybridization capture method was developed and applied to explore the feasibility of retrieving complete plastid genomes from Guibourtia sapwood and heartwood specimens stored in a xylarium (wood collection). We then carried out forensic identification and phylogenetic analyses of Guibourtia within the subfamily Detarioideae. This study is the first to successfully retrieve high-quality plastid genomes from xylarium specimens, with 76.95-99.97% coverage. The enrichment efficiency, sequence depth, and coverage of plastid genomes from sapwood were 16.73 times, 70.47 times and 1.14 times higher, respectively, than those from heartwood. Although the DNA capture efficiency of heartwood was lower than that of sapwood, the hybridization capture method used in this study is still suitable for heartwood DNA analysis. Based on the complete plastid genome, we identified six endangered or commonly traded Guibourtia woods at the species level. This technique also has the potential for geographical traceability, especially for Guibourtia demeusei and Guibourtia ehie. Meanwhile, Bayesian phylogenetic analysis suggested that these six Guibourtia species diverged from closely related species within the subfamily Detarioideae ca. 18 Ma during the Miocene. The DNA reference database established based on the xylarium specimens provides admissible evidence for diversity conservation and evolutionary analyses of endangered Guibourtia species.