文献类型：期刊文献

英文题名：METHIONINE SYNTHASE1 Is Involved in Chromatin Silencing by Maintaining DNA and Histone Methylation

作者：Yan, Xiaojing[1,2] Ma, Liang[3] Pang, Hongying[1,2] Wang, Ping[3] Liu, Lei[3] Cheng, Yanxia[1,2] Cheng, Jinkui[3] Guo, Yan[3] Li, Quanzi[1,2]

第一作者：闫晓婧

通信作者：Li, QZ[1];Li, QZ[2]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China;[3]China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing 100193, Peoples R China

年份：2019

卷号：181

期号：1

起止页码：249-261

外文期刊名：PLANT PHYSIOLOGY

收录：;Scopus(收录号：2-s2.0-85071784818);WOS:【SCI-EXPANDED(收录号:WOS:000483941300023)】；

基金：This work was supported by grants from the Fundamental Research Funds of Chinese Academy of Forestry (CAFYBB2017ZY001), The Opening Scientific Program of State Key Laboratory of Plant Physiology and Biochemistry (SKLPPBKF1809), and the Natural Science Foundation of China (31801067).

语种：英文

摘要：DNA methylation and histone modification are important epigenetic marks that coregulate gene expression and genome stability. To identify factors involved in chromatin silencing, we carried out a forward genetic screen for mutants that release the silenced Pro-35S:LUCIFERASE (35SP-LUC) in Arabidopsis (Arabidopsis thaliana). We identified an epigenetic regulator, METHIONINE SYNTHASE1 (ATMS1), which catalyzes the synthesis of methionine (Met) in the one-carbon metabolism pathway. The ATMS1 mutation releases the silenced 35SP-LUC and the majority of endogenous genes and transposons. The effect of ATMS1 on chromatin silencing is related to decreased levels of DNA methylation (CG, CHG, and CHH) and histone-3 lysine-9 dimethylation. The ATMS1 mutation caused a significant decrease in the ratio of S-adenosylmethionine to S-adenosylhomocysteine. Exogenous application of Met rescued the phenotype of atms1-1. ATMS1 plays a predominant role in DNA and histone methylations among the three Met synthetase homologs. These results suggest that ATMS1 is required for DNA and histone methylations through its function in the one-carbon metabolism pathway, indicating the complex interplay between metabolism and epigenetic regulation.