文献类型：期刊文献

中文题名：松针叶绿素-胡萝卜素软膏体外清除自由基及体内抗氧化作用的研究

英文题名：Study on free radial scavenging capacity and antioxidation of chlorophyll-carotene paste from pine needles in vitro and in vivo

作者：郑光耀[1] 何玲[2] 薄采颖[1] 谢衡[3] 刘冬芹[2] 谢学容[3]

第一作者：郑光耀

机构：[1]中国林业科学研究院林产化学工业研究所.生物质化学利用国家工程实验室;[2]国家林业局林产化学工程重点开放性实验室;[3]中国药科大学药理学教研室

年份：2013

卷号：24

期号：4

起止页码：815-817

中文期刊名：时珍国医国药

外文期刊名：Lishizhen Medicine and Materia Medica Research

收录：北大核心:【北大核心2011】；CSCD:【CSCD_E2013_2014】；

基金：科技部农业科技成果转化资金项目(No.2008GB24320412);2010年教育部"新世纪优秀人才"支持计划资助项目(No.NCET-10-0817);国家"十二五"重大新药创制专项(No.2012ZX09504001-001)

语种：中文

中文关键词：松针;提取物;自由基;抗氧化

外文关键词：Pine needles;Extract;Free radical;Antioxidation

分类号：R284.2

摘要：目的研究松针叶绿素-胡萝卜素软膏体外清除自由基和体内抗氧化作用。方法通过测定松针叶绿素-胡萝卜素软膏对羟自由基(·OH)、超氧阴离子自由基(O2-·)和DPPH自由基(DPPH·)的清除率,观察松针叶绿素-胡萝卜素软膏体外清除自由基能力;通过测定大鼠血清、肝匀浆和心匀浆超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量变化,观察松针叶绿素-胡萝卜素软膏体内抗氧化作用。结果松针叶绿素-胡萝卜素软膏对·OH、O2-·、DPPH.均具有明显的清除能力,其IC50分别为0.53,0.23,0.56 mg/ml;能显著提高大鼠血清、肝脏和心脏中SOD、GSH-Px活性和降低MDA含量。结论松针叶绿素-胡萝卜素软膏在体内外具有良好的抗氧化活性,为松针叶绿素-胡萝卜素软膏在食品和药物中的应用提供实验依据。
Objective To study on free radial scavenging capacity and antioxidation of chlorophyll-carotene paste from pine needles. Methods The free radical scavenging capacity of chlorophyll-carotene paste was evaluated by measuring the scavenging rate of hydroxyl radical,superoxide radical and DPPH radical in vitro.The antioxidant activity of chlorophyll-carotene paste was analyzed by measuring SOD,GSH-Px and MDA concentrations in serum,liver and heart of rat in vivo. Results The chlorophyll-carotene paste showed high scavenging capacity against the hydroxyl,superoxide and DPPH radicals with IC50 values of 0.53,0.23 and 0.56 mg/ml,respectively.The chlorophyll-carotene paste could significantly increase the activities of SOD and GSH-Px while decrease the content of MDA in serum,liver and heart of rat. Conclusion Chlorophyll-carotene paste from pine needles might be a valuable antioxidant of natural source that could be applicable in the food and medicine industry.