文献类型：期刊文献

英文题名：Genome-wide and functional analysis of late embryogenesis abundant (LEA) genes during dormancy and sprouting periods of kernel consumption apricots (P. armeniaca L. x P. sibirica L.)

作者：Li, Shaofeng[1] Wuyun, Ta-na[2] Wang, Lin[2] Zhang, Jianhui[3] Tian, Hua[1] Zhang, Yaodan[1] Wang, Shaoli[1] Xia, Yongxiu[1] Liu, Xue[1] Wang, Ning[1] Lv, Fenni[4,5] Xu, Jihuang[6] Tang, Zhimin[7]

第一作者：李少锋

通信作者：Xu, JH[1];Tang, ZM[2]

机构：[1]Chinese Acad Forestry, Expt Ctr Forestry North China, Natl Permanent Sci Res Base Warm Temperate Zone Fo, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Nontimber Forestry Res & Dev Ctr, State Key Lab Tree Genet & Breeding, Zhengzhou 450003, Peoples R China;[3]Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China;[4]Inst Bot, Jiangsu Key Lab Res & Utilizat Plant Resources, Nanjing 210014, Jiangsu, Peoples R China;[5]Chinese Acad Sci, Nanjing Bot Garden Mem Sun Yat Sen, Nanjing 210014, Peoples R China;[6]Chinese Acad Forestry, Expt Ctr Trop Forestry, Pingxiang 532600, Peoples R China;[7]Chinese Acad Forestry, Inst Ecol Conservat & Restorat, Beijing 100093, Peoples R China

年份：2024

卷号：279

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001319148800001)】；

基金：We are grateful to Dr Zanmin Hu (Institute of Genetics and Devel-opmental Biology, Chinese Academy of Sciences) for constructive sug-gestions and helpful advice. This work was supported by the Fundamental Research Funds of CAF (CAFYBB2018MA003 and CAFYBB2020QD001) , the National Natural Science Foundation of China (31770705 and 31400570) .

语种：英文

外文关键词：Genome-wide identification; Cold resistance; Flower bud dormancy; Kernel consumption apricots (P. armeniaca L. x P. sibirica L.); Late embryogenesis abundant (LEA) protein

摘要：Late embryogenesis abundant (LEA) proteins play a crucial role in protecting cells from stress, making them potential contributors to abiotic stress tolerance. This study focuses on apricot (P. armeniaca L. x P. sibirica L.), where a comprehensive genome-wide analysis identified 54 LEA genes, categorized into eight subgroups based on phylogenetic relationships. Synteny analysis revealed 14 collinear blocks containing LEA genes between P. armeniaca x P. sibirica and Arabidopsis thaliana, with an additional 9 collinear blocks identified between P. armeniaca x P. sibirica and poplar. Examination of gene structure and conserved motifs indicated that these subgroups exhibit consistent exon-intron patterns and shared motifs. The expansion and duplication of LEA genes in P. armeniaca x P. sibirica were driven by whole-genome duplication (WGD), segmental duplication, and tandem duplication events. Expression analysis, utilizing RNA-seq data and quantitative real-time RT-PCR (qRTPCR), indicated induction of PasLEA2-20, PasLEA3-2, PasLEA6-1, Pasdehydrin-3, and Pasdehydrin-5 in flower buds during dormancy and sprouting phases. Coexpression network analysis linked LEA genes with 15 cold-resistance genes. Remarkably, during the four developmental stages of flower buds in P. armeniaca x P. sibirica - physiological dormancy, ecological dormancy, sprouting period, and germination stage - the expression patterns of all PasLEAs coexpressed with cold stress-related genes remained consistent. Protein-protein interaction networks, established using Arabidopsis orthologs, emphasized connections between PasLEA proteins and cold resistance pathways. Overexpression of certain LEA genes in yeast and Arabidopsis conferred advantages under cold stress, including increased pod length, reduced bolting time and flowering time, improved survival and seed setting rates, elevated proline accumulation, and enhanced antioxidative enzymatic activities. Furthermore, these overexpressed plants exhibited upregulation of genes related to flower development and cold resistance. The Y1H assay confirmed that PasGBF4 and PasDOF3.5 act as upstream regulatory factors by binding to the promoter region of PasLEA3-2. PasDOF2.4, PasDnaJ2, and PasAP2 were also found to bind to the promoter of Pasdehydrin3, regulating the expression levels of downstream genes. This comprehensive study explores the evolutionary relationships among PasLEA genes, protein interactions, and functional analyses during various stages of dormancy and sprouting in P. armeniaca x P. sibirica. . It offers potential targets for enhancing cold resistance and manipulating flower bud dormancy in this apricot hybrid.