文献类型：期刊文献

英文题名：Long read sequencing of Toona sinensis (A. Juss) Roem: A chromosome-level reference genome for the family Meliaceae

作者：Ji, Yun-Tao[1] Xiu, Zhihui[2] Chen, Chun-Hai[2] Wang, Youru[3] Yang, Jing-Xia[1] Sui, Juan-Juan[1] Jiang, San-Jie[2] Wang, Ping[2] Yue, Shao-Yun[1] Zhang, Qian-Qian[2] Jin, Ji-liang[1] Wang, Guo-Shu[4] Wei, Qian-Qian[2] Wei, Bing[1] Wang, Juan[1] Zhang, Hai-Lin[2] Zhang, Qiu-Yan[1] Liu, Jun[5] Liu, Chang-Jin[6] Jian, Jian-Bo[2,7,8] Qu, Chang-Qing[1]

第一作者：Ji, Yun-Tao

通信作者：Qu, CQ[1];Jian, JB[2];Liu, J[3];Liu, CJ[4]

机构：[1]Fuyang Normal Univ, Biol & Food Engn Sch, Engn Technol Res Ctr Antiaging Chinese Herbal Med, Fuyang, Peoples R China;[2]BGI Shenzhen, BGI Genom, Shenzhen, Peoples R China;[3]Hubei Normal Univ, Hubei Engn Res Ctr Typ Wild Vegetables Breeding &, Huangshi, Hubei, Peoples R China;[4]Forestry Nursery Taihe Cty, Fuyang, Peoples R China;[5]Chinese Acad Forestry, Res Inst Subtrop Forestry, Fuyang, Peoples R China;[6]Tianjin Univ Sci & Technol, Sch Food Sci & Technol, State Key Lab Food Nutr & Safety, Tianjin, Peoples R China;[7]Tech Univ Denmark, Dept Biotechnol & Biomed, Lyngby, Denmark;[8]BGI Shenzhen, Minist Agr, Key Lab Genom, Shenzhen, Peoples R China

年份：2021

卷号：21

期号：4

起止页码：1243-1255

外文期刊名：MOLECULAR ECOLOGY RESOURCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000612233000001)】；

基金：New rural development research institute grant project, Grant/Award Number: XNC 201705; Deep processing technology research and development team of Toona sinensis, Grant/Award Number: XDHX201726, XDHX201736, XDHXPT201703 and XDHXTD201702; Anhui University Research Innovation Platform Construction Project; Key projects of Natural Science Research of Education Department of Anhui Province, Grant/Award Number: KJ2019A0516, KJ2020A0520,KJ2019A0527; University collaborative innovation project of Anhui Province, Grant/Award Number: GXXT-2019-049,GXXT-2019-009; Anhui Province Science and Technology Major Special Project, Grant/Award Number: 202003a06020020 and 201904f06020018; National Natural Science Foundation of China, Grant/Award Number: 52003053; Natural Science Foundation of Anhui Province, Grant/Award Number: 2008085QE275

语种：英文

外文关键词：evolution; Hi-C; Nanopore; Toona sinensis; whole genome duplication

摘要：Chinese mahogany (Toona sinensis) is a woody plant that is widely cultivated in China and Malaysia. Toona sinensis is important economically, including as a nutritious food source, as material for traditional Chinese medicine and as a high-quality hardwood. However, the absence of a reference genome has hindered in-depth molecular and evolutionary studies of this plant. In this study, we report a high-quality T. sinensis genome assembly, with scaffolds anchored to 28 chromosomes and a total assembled length of 596 Mb (contig N50 = 1.5 Mb and scaffold N50 = 21.5 Mb). A total of 34,345 genes were predicted in the genome after homology-based and de novo annotation analyses. Evolutionary analysis showed that the genomes of T. sinensis and Populus trichocarpa diverged similar to 99.1-103.1 million years ago, and the T. sinensis genome underwent a recent genome-wide duplication event at similar to 7.8 million years and one more ancient whole genome duplication event at similar to 71.5 million years. These results provide a high-quality chromosome-level reference genome for T. sinensis and confirm its evolutionary position at the genomic level. Such information will offer genomic resources to study the molecular mechanism of terpenoid biosynthesis and the formation of flavour compounds, which will further facilitate its molecular breeding. As the first chromosome-level genome assembled in the family Meliaceae, it will provide unique insights into the evolution of members of the Meliaceae.