文献类型：期刊文献

英文题名：Two Burkholderia strains from nodules of Dalbergia odorifera T. Chen in Hainan Island, southern China

作者：Lu, J. K.[1] He, X. H.[2,3,4] Huang, L. B.[5] Kang, L. H.[1] Xu, D. P.[1]

第一作者：陆俊锟

通信作者：Xu, DP[1]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Guangdong, Peoples R China;[2]Edith Cowan Univ, Ctr Ecosyst Management, Sch Nat Resources, Joondalup, WA 6027, Australia;[3]Univ Western Australia, State Ctr Excellence Ecohydrol, Crawley, WA 6009, Australia;[4]Univ Western Australia, Sch Plant Biol, Crawley, WA 6009, Australia;[5]Univ Queensland, Ctr Mined Land Rehabil, Sustainable Minerals Inst, Brisbane, Qld 4072, Australia

年份：2012

卷号：43

期号：4

起止页码：397-409

外文期刊名：NEW FORESTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000305889900001)】；

基金：This work was financially supported by projects from the Guangdong Forestry Science and Technology for Creativity and Special Fund (2009KJCX002-01), the State Forestry Bureau 948 Technology Improvement Program (2008-04-02) and National Natural Science Foundation of China (31170582).

语种：英文

外文关键词：Betaproteobacteria; Burkholderia; Dalbergia odorifera; 16S rRNA; 16S-23S; Gene internally transcribed spacer; Symbiosis

摘要：Two Burkholderia strains 8111 and 8201 were isolated from root nodules of Dalbergia odorifera, an endemic woody legume in southern China. Phylogenetic analysis of 16S rRNA gene and 16S-23S gene intergenic spacer (ITS) showed that these two bacterial strains were closely related to Burkholderia cepacia and they were also similar in carbon source utilization using Biolog GN2 plate tests. The DNA G+C content of strains 8111 and 8201 were 65.8 and 65.5 mol%. Inoculation tests demonstrated that both strains 8111 and 8201 formed functional root nodules in their original host D. odorifera, and significantly enhanced plant growth (as measured by plant biomass and nitrogen content), compared to the no-inoculated control plants. Sequencing of 16S rRNA gene in nodules of D. odorifera seedlings inoculated with strains 8111 and 8201 confirmed their identity. However, these two strains did not induce root nodulation in Acacia auriculiformis and Erythrophleum fordii. This implies that the nodulation capacity between Burkholderia strains 8111 or 8201 and their legume hosts may be specific. Our results show that both Burkholderia strains 8111 and 8201 are able to form functional nodules on D. odorifera and are potentially beneficial inoculants for seedling propagation to be used in large scale D. odorifera plantations.