文献类型：期刊文献

中文题名：基于均匀设计对黄精不定芽增殖培养的研究

英文题名：Research on the Rhizome Bud in Vitro Culture Of Polygonatum cyrtonema Hua Based on the Uniform Design Model

作者：周新华[1] 肖智勇[2] 王丽云[1] 陈传松[1] 钟文斌[1] 钟秋平[1]

第一作者：周新华

机构：[1]中国林业科学研究院亚热带林业实验中心;[2]宜春市林业科学研究所

年份：2014

卷号：42

期号：31

起止页码：10909-10911

中文期刊名：安徽农业科学

外文期刊名：Journal of Anhui Agricultural Sciences

基金：中央级公益性科研院所基本科研业务费专项资金(CAF-YBB2-012002)

语种：中文

中文关键词：多花黄精;均匀设计;组织培养;芽增殖

外文关键词：Polygonatum crytonema Hua;Uniform design;Tissue culture;Bud propagation

分类号：S567

摘要：[目的]筛选出最适合离体多花黄精不定芽增殖的培养基配方。[方法]以多花黄精带芽根茎为外植体,在激素种类、水平数较多的情况下,采用了均匀设计及其分析方法筛选出最适合黄精不定芽增殖的培养基配方。[结果]最佳培养基配方为MS+KT 1.49 mg/L+NAA 0.29 mg/L+琼脂0.7%+蔗糖3%(pH=5.8),平均增殖系数高达7.09以上。[结论]研究结果为多花黄精规模化、工厂化育苗提供理论和技术支撑。
[ Objective ] The research aimed to select the optimal media formulations of the rhizome bud [ Method ] Taking the Polygonatum cyrtonema Hun with rhizome and buds as explants, the optimal media formulations of the adventitious bud propagation in the Vitro Culture of Polygo- natum cyrtonema Hun was selected by the uniform design and analysis methods under the cases of many hormone kinds and levels. [ Result]The results showed that MS ＋ KT 1.49 mg/L ＋ NAA 0.29 mg/L ＋ Agar 0.7% ＋ Sugar 3% （ pH = 5.8 ） wsa the best medium for the adventitious buds induction. The average buds number induced by a bud is 7.09. [ Conclusion] The research results can provide the theoretical and technical support for the cyrtonema scale and industrialized breeding of Polygonatum cyrtonema Hun.