文献类型：期刊文献

英文题名：Non-Additive Gene Expression in Carbon and Nitrogen Metabolism Drives Growth Heterosis in Populus deltoides

作者：Zhang, Jing[1,2] Zhang, Weixi[1,2] Ding, Changjun[1,2] Zhao, Jun[3] Su, Xuehui[4] Yuan, Zhengsai[1,2] Chu, Yanguang[1,2] Huang, Qinjun[1,2] Su, Xiaohua[1,2,5]

通信作者：Ding, CJ[1];Su, XH[1];Ding, CJ[2];Su, XH[2];Su, XH[3]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Breeding & Cultivat State Fores, Beijing, Peoples R China;[3]Jiaozuo Univ, Jiaozuo, Peoples R China;[4]Jiaozuo Acad Agr & Forestry Sci, Jiaozuo, Peoples R China;[5]Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing, Peoples R China

年份：2025

外文期刊名：PLANT CELL AND ENVIRONMENT

收录：;Scopus(收录号：2-s2.0-85214358595);WOS:【SCI-EXPANDED(收录号:WOS:001392974600001)】；

基金：This research was supported by the STI 2030-Major Projects (Grant No. 2023ZD0405603), the National Natural Science Foundation of China (Grant No. 31870662), the Basic Research Fund of CAF (Grant No. CAFYBB2023QB003), the National Forestry and Grassland Administration Science and Technology Innovation Development Project (Grant No. 2024132027) and the National Key Research and Development Program of China (Grant No. 2021YFD2201205).

语种：英文

外文关键词：carbon and nitrogen metabolism; growth heterosis; non-additive expression patterns; Populus Deltoides; transcriptome sequencing

摘要：Growth heterosis is crucial for Populus deltoides breeding, a key industrial-timber and ecological-construction tree species in temperate regions. However, the molecular mechanisms underlying carbon (C)-nitrogen (N) metabolism coordination in regulating growth heterosis remain unclear. Herein high-hybrids of P. deltoides exhibited high-parent heterosis and mid-parent heterosis in growth traits and key enzymes of C-N metabolism. In hybrids, gene expression patterns were mainly biased toward female parent. Parental contribution to growth heterosis in P. deltoides is differentiation, rather than absolute maternal or paternal dominance contributions. Parental genes were predominantly and dynamically inherited in a non-additive manner, mainly with dominant expression patterns. A total of 44 non-additive genes associated with photosynthetic C fixation, starch and sucrose metabolism, sucrose transport, photorespiration, and nitrogen metabolism coregulated growth heterosis by coordinating C-N metabolism. Growth-regulating factors 4 interacted with DELLA genes to promote growth by enhancing this coordination. Additionally, five critical genes were identified. Briefly, the above genes in high-hybrids improved photosynthesis and N utilisation by regulating carbohydrate accumulation and enzyme activity, while reducing respiratory energy consumption, thereby providing more energy for growth and promoting growth heterosis. Our findings offer new insights and theoretical basis for deep understanding genetic and molecular regulation mechanisms of tree heterosis and its application in precision hybrid breeding.