文献类型：期刊文献

英文题名：Identification of novel cis-elements bound by BpIMYB46 involved in abiotic stress responses and secondary wall deposition

作者：Guo, Huiyan[1] Wang, Liuqiang[2] Yang, Chuanping[1] Zhang, Yiming[1] Zhang, Chunrui[1] Wang, Chao[1]

第一作者：Guo, Huiyan

通信作者：Wang, C[1]

机构：[1]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin 150040, Heilongjiang, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Res Inst Forestry, Beijing 100091, Peoples R China

年份：2018

卷号：60

期号：10

起止页码：1000-1014

外文期刊名：JOURNAL OF INTEGRATIVE PLANT BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000446451000007)】；

基金：The authors thank Dr. Li Tan for the suggestion on the manuscript revision. This study was supported by two grants from the National Natural Science Foundation of China (31470671 and 31700587).

语种：英文

摘要：Transcription factors (TFs) play vital roles in various biological processes by binding to cis-acting elements to control expressions of their target genes. The MYB TF BpIMYB46, from Betula platyphylla, is involved in abiotic stress responses and secondary wall deposition. In the present study, we used a TF-centered yeast one- hybrid technology (TF-centered Y1H) to identify the cis- acting elements bound by BpIMYB46. We screened a shortinsert random library and identified three cis-elements bound by BpIMYB46: an E-box (CA(A/T/C)(A/G/C)TG) and two novel motifs, a TC-box (T(G/A)TCG(C/G)) and a GT-box (A(G/T)T(A/C)GT(T/G)C). Chromatin immunoprecipitation (ChIP) and effector-reporter coexpression assays in Nicotiana tabacum confirmed binding of BpIMYB46 to the TC-box, GT-box, and E-box motifs in the promoters of the phenylalanine ammonia lyase (PAL), peroxidase (POD), and superoxide dismutase (SOD) genes, which function in abiotic stress tolerance and secondary wall biosynthesis. This finding improves our understanding of potential regulatory mechanisms in the response to abiotic stress and secondary wall deposition of BpIMYB46 in B. platyphylla.