文献类型：期刊文献

英文题名：Analysis of culm elongation in photoheterotrophic status of Dendrocalamus sinicus by comparative proteomics

作者：Cui, Kai[1] Wang, Haiying[1] He, Caiyun[2] Sun, Di[3] Zhang, Junpei[2] Liao, Shengxi[1] Cui, Yongzhong[1] Li, Li[1]

通信作者：Liao, SX[1]

机构：[1]Chinese Acad Forestry, Res Inst Resources Insects, Kunming 650224, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China;[3]Texas A&M Univ, Inst Plant Genom & Biotechnol, College Stn, TX 77843 USA

年份：2017

卷号：31

期号：2

起止页码：687-704

外文期刊名：TREES-STRUCTURE AND FUNCTION

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000398106900025)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant No. 31300501), the Fundamental Research Funds for the Central Nonprofit Research Institution of CAF (Grant No. CAFYBB2014QB020 and riricaf2013002M), and the China Scholarship Council (201503270020).

语种：英文

外文关键词：Culm elongation; Proteome; Gene expression; Starch biosynthesis

摘要：Dendrocalamus sinicus is known as the largest bamboo species in the world, characterized by huge woody culms with rapid growth rate. During culm elongation, leaves are not yet shaped and culms are covered by non-transparent overlapping sheaths, forming a photo-heterotrophic status in which culms rapidly complete their own growth. To elucidate this phenomenon, proteome analysis as well as enzyme activity were performed. In terms of the enzyme activity, we found significant differences among internodes for adenosine diphosphoglucose pyrophosphorylase, starch synthase, and starch branching enzyme, implying existence of a robust response of starch synthesis throughout culm elongation. At the protein level, 2-DE combined with MALDI-TOF/TOF MS analysis showed 36 differentially accumulated proteins among internodes. Of which, the largest group is the cluster for energy metabolism (27.78%), followed by central intermediary metabolism (22.22%), which suggests that carbohydrate metabolism dominates the process of culm elongation. Especially, four proteins related to the glycolysis pathway were differentially expressed. In addition, a protochlorophyllide reductase associated with chlorophyllide synthesis was down-regulated from basal to top internodes. This implies that culms belong to photosynthetic tissue and woody tissue photosynthesis is involved in the culm elongation. In addition, malate dehydrogenase related to TCA cycle was up-regulated from tender to mature tissues, which indicates that it plays a vital role in energy generation. An inconsistency between the transcript and protein level was found, suggesting that post-transcriptional events are involved in the culm elongation. These results are helpful to disclose the multiple mechanism of culm elongation of D. sinicus.