文献类型：期刊文献

英文题名：Genome-Wide Identification and Characterization of the NF-YA Gene Family and Its Expression in Response to Different Nitrogen Forms in Populus x canescens

作者：Zhou, Jing[1] Yang, Lingyu[1] Chen, Xin[1] Zhou, Mengyan[1] Shi, Wenguang[1] Deng, Shurong[1] Luo, Zhibin[1]

第一作者：周婧

通信作者：Zhou, J[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Silviculture,Natl Forestry & Grassland Ad, Beijing 100091, Peoples R China

年份：2022

卷号：23

期号：19

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000867764900001)】；

基金：This work was supported by the Fundamental Research Funds of CAF (grant no. CAFYBB 2022SY007) and the National Natural Science Foundation of China (grant no. 32171739).

语种：英文

外文关键词：Populus x canescens; NF-YA; gene family; expression analysis; nitrogen

摘要：The NF-YA gene family is a class of conserved transcription factors that play important roles in plant growth and development and the response to abiotic stress. Poplar is a model organism for studying the rapid growth of woody plants that need to consume many nutrients. However, studies on the response of the NF-YA gene family to nitrogen in woody plants are limited. In this study, we conducted a systematic and comprehensive bioinformatic analysis of the NF-YA gene family based on Populus x canescens genomic data. A total of 13 PcNF-YA genes were identified and mapped to 6 chromosomes. According to the amino acid sequence characteristics and genetic structure of the NF-YA domains, the PcNF-YAs were divided into five clades. Gene duplication analysis revealed five pairs of replicated fragments and one pair of tandem duplicates in 13 PcNF-YA genes. The PcNF-YA gene promoter region is rich in different cis-acting regulatory elements, among which MYB and MYC elements are the most abundant. Among the 13 PcNF-YA genes, 9 contained binding sites for P. x canescens miR169s. In addition, RT-qPCR data from the roots, wood, leaves and bark of P. x canescens showed different spatial expression profiles of PcNF-YA genes. Transcriptome data and RT-qPCR analysis showed that the expression of PcNF-YA genes was altered by treatment with different nitrogen forms. Furthermore, the functions of PcNF-YA genes in transgenic poplar were analyzed, and the potential roles of PcNF-YA genes in the response of poplar roots to different nitrogen forms were revealed, indicating that these genes regulate root growth and development.