文献类型：期刊文献

中文题名：山核桃干腐病病原菌的鉴定

英文题名：Identification of the pathogens causing stem canker on Carya cathayensis

作者：王璇[1,2] 马良进[1] 吕全[2] 孟娴静[2] 张星耀[2]

第一作者：王璇

机构：[1]浙江农林大学林业与生物技术学院;[2]中国林业科学研究院森林生态环境与保护研究所/国家林业局森林保护学重点实验室

年份：2014

卷号：31

期号：2

起止页码：238-245

中文期刊名：浙江农林大学学报

外文期刊名：Journal of Zhejiang A & F University

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD_E2013_2014】；

基金：中央级公益性科研院所基本科研业务费专项资金项目(CAFYBB2011005);浙江省农业"生物三药"产业重点科技创新团队资助项目(2009R50G2010001-5)

语种：中文

中文关键词：森林保护学;经济林学;溃疡病;葡萄座腔菌科;茶藨子葡萄座腔菌;系统发育;山核桃

外文关键词：forest protection; cash forestry; canker; Botryosphaeriaceae; Botryosphaeria dothidea; phylogeny;Carya cathayensis

分类号：S759.3;S763.1

摘要：葡萄座腔菌科Botryosphaeriaceae真菌是很多木本植物溃疡病和枯梢病的重要病原菌。已有报道认为:山核桃干腐病病原菌为茶藨子葡萄座腔菌Botryosphaeria dothidea,但对于葡萄座腔菌科其他真菌与山核桃干腐病的关系还没有深入研究。分离得到了山核桃干腐病相关病原菌,通过比较其形态学特征和核糖体DNA内转录间隔区(rDNA ITS)序列特征,将分离得到的供试菌株分为3组。根据形态学特征和基于ITS序列的系统发育分析,3组菌株分别鉴定为Botryosphaeria dothidea,B.fabicercianum和B.obtusa。其中B.dothidea为优势菌株,分离频率为71.42%,B.fabicercianum和B.obtusa分离频率分别为14.28%。经致病性测定,上述分离菌株均能引起健康山核桃Carya cathayensis枝条发病,但致病性存在差异。在山核桃上发现的B.obtusa和B.fabicercianum为首次报道。
Species of the Botryosphaeriaceae family are important pathogens causing cankers and die-back on many woody plants. In previous studies Botryosphaeria dothidea has commonly been reported as a pathogen of Chinese hickory （Carya cathayensis ） canker; however, it was unclear whether other species of Botryosphaeri- aceae were also related to hickory canker. In one study, several species of Botryosphaeriaceae were isolated from Chinese hickory, and then they were identified based on anamorphic morphology and rDNA Internal Tran- scribed Spacer （ITS） sequences. A phylogentic analysis indicated that these isolates formed three clades and were identified as B. dothidea, B. fabicercianum, and B. obtusa with isolation rates of B. dothidea （71.42%） predominant and B. fabicercianum and B. obtusa being much lower （14.28%）. In pathogenicity testing, all three pathogens caused stem cankers on Chinese hickory but had different degrees of pathogenicity. B. obtusa and B. fabicercianum were first reported as a pathogen of Carya cathayensis canker.