文献类型：期刊文献

英文题名：A poly(5-indolylboronic acid) based molecular imprint doped with carbon dots for fluorometric determination of glucose

作者：Zhou, Xi[1,2] Gao, Xuexia[1] Liu, Meihong[1] Wang, Chunpeng[1,2] Chu, Fuxiang[1,2]

通信作者：Chu, FX[1];Chu, FX[2]

机构：[1]Chinese Acad Forestry, Inst Chem Ind Forestry Prod, Natl Engn Lab Biomass Chem Utilizat, Key Lab Biomass Energy & Mat Jiangsu Prov, Nanjing 210042, Jiangsu, Peoples R China;[2]Chinese Acad Forestry, Inst Forest New Technol, Beijing 100091, Peoples R China

年份：2017

卷号：184

期号：10

起止页码：4175-4181

外文期刊名：MICROCHIMICA ACTA

收录：;EI(收录号：20242616334582);Scopus(收录号：2-s2.0-85027960363);WOS:【SCI-EXPANDED(收录号:WOS:000410517400064)】；

基金：This work was supported by the Fundamental Research Funds for the Central Non-profit Research Institution of CAF (CAFYBB2017ZX003), the Fundamental Research Funds of Research Institute of Forest New Technology (CAFYBB2017SY033).

语种：英文

外文关键词：Hybrid nanomaterial; Aggregation; Cis-diols; Quenching

摘要：A hybrid nanomaterial was synthesized that consists of poly(5-indolylboronic acid) acting as a molecular imprint (MIP), and of fluorescent carbon dots (CDs). The combination of CDs and MIP endowed the hybrid with stable fluorescence and template selectivity. The addition of glucose causes aggregation and quenching of fluorescence. Aggregation is due to the formation of covalent bonds between the cis-diols of glucose molecules and boronic acid side groups. The fluorometric signal, obtained at excitation/emission wavelengths of 350/450 nm, varies in the 5 to 750 mu M glucose concentration range, with a 0.5 mu M detection limit. The assay is not interfered by various biomolecules including fructose and was successful applied to the determination of glucose in (spiked) serum. In our perception, the results make this glucose assay an attractive new tool for clinical applications.