文献类型：期刊文献

英文题名：Suppression of highly efficient cadmium translocation by exogenous gibberellin provides insights into the molecular regulation mechanism of hyperaccumulation in Sedum plumbizincicola

作者：Zhang, Yixin[1,2] Wu, Xiaotong[3] Mo, Yanlan[4] Han, Liyuan[1] Peng, Xin[5] Sun, Zhenyuan[1] Xu, Wenzhong[2,6,7]

第一作者：Zhang, Yixin

通信作者：Sun, ZY[1];Xu, WZ[2]

机构：[1]Chinese Acad Forestry, State Forestry Adm, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat,Res Inst Forestry, Beijing 100091, Peoples R China;[2]Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China;[3]Southeast Univ, Sch Energy & Environm, Nanjing 210096, Peoples R China;[4]Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Peoples R China;[5]Hunan Agr Univ, Changsha 410128, Peoples R China;[6]China Natl Bot Garden, Beijing 100093, Peoples R China;[7]Univ Chinese Acad Sci, Beijing 100049, Peoples R China

年份：2023

卷号：209

外文期刊名：ENVIRONMENTAL AND EXPERIMENTAL BOTANY

收录：;Scopus(收录号：2-s2.0-85150348804);WOS:【SCI-EXPANDED(收录号:WOS:000957556600001)】；

基金：This work was supported by the National Natural Science Foundation of China (32172666) .

语种：英文

外文关键词：Sedum plumbizincicola; Cadmium; Transport; Gibberellin; Transcriptome sequencing

摘要：Sedum plumbizincicola is a hyperaccumulating species with highly efficient uptake, translocation and distribution system for cadmium (Cd), a non-essential and toxic heavy metal. To understand the molecular regulation mechanisms of Cd transport and accumulation in this hyperaccumulator is still a challenge. In this study, we observed that exogenous gibberellin A3 (GA3) significantly reduced Cd loading efficiency from root to shoot and uploading efficiency from stem to leaves in S. plumbizincicola under Cd treatments, which provided us a remarkable opportunity to study the signaling mechanisms for regulation of highly efficient Cd transport and translocation in S. plumbizincicola. Here, we constructed a de novo full-length transcriptome database with the integration of full-length and next-generation transcriptome sequences for Cd-treated S. plumbizincicola with or without exogenous GA3 application. From this database, we identified 1461 differentially expressed genes (DEGs), which were related to solute transport, cell wall metabolism and signal transduction, and most of them were down-regulated by the exogenous application of gibberellin. In particularly, a substantial number of the downregulated genes belong to calmodulin-like (CML) proteins, calcium-dependent protein kinase, calcium-permeable channel, mitochondrial calcium uniporter (MCU) and Na+/Ca2+ exchanger-like proteins (NCL/EF-CAX). These results indicated that calcium homeostasis and signal transduction may play an important role in the regulation of highly efficient translocation of cadmium in hyperaccumulator S. pumbizincicola.