文献类型：期刊文献

英文题名：Genome-Wide Characterization of the sHsp Gene Family in Salix suchowensis Reveals Its Functions under Different Abiotic Stresses

作者：Li, Jianbo[1,2] Zhang, Jin[1,3] Jia, Huixia[1,3] Yue, Zhiqiang[1] Lu, Mengzhu[1,3,4] Xin, Xuebing[2] Hu, Jianjun[1,3,4]

通信作者：Hu, JJ[1];Xin, XB[2];Hu, JJ[3];Hu, JJ[4]|[a000547af0b9516fa7bc5]辛学兵;

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Expt Ctr Forestry North China, Beijing 102300, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat Natl Forestry &, Beijing 100091, Peoples R China;[4]Nanjing Forestry Univ, Collaborat Innovat Ctr Sustainable Forestry South, Nanjing 210037, Jiangsu, Peoples R China

年份：2018

卷号：19

期号：10

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000448951000401)】；

基金：This work was supported by the National Nonprofit Institute Research Grant of the Chinese Academy of Forestry (CAFYBB2017ZY008), (CAFYBB2018ZY001-9), the National Natural Science Foundation of China (31570669) and (31800570).

语种：英文

外文关键词：small heat shock proteins (sHsp); phylogenetic analysis; gene structure; expression analysis; Salix suchowensis

摘要：Small heat shock proteins (sHsps) function mainly as molecular chaperones that play vital roles in response to diverse stresses, especially high temperature. However, little is known about the molecular characteristics and evolutionary history of the sHsp family in Salix suchowensis, an important bioenergy woody plant. In this study, 35 non-redundant sHsp genes were identified in S. suchowensis, and they were divided into four subfamilies (C, CP, PX, and MT) based on their phylogenetic relationships and predicted subcellular localization. Though the gene structure and conserved motif were relatively conserved, the sequences of the Hsp20 domain were diversified. Eight paralogous pairs were identified in the Ssu-sHsp family, in which five pairs were generated by tandem duplication events. Ka/Ks analysis indicated that Ssu-sHsps had undergone purifying selection. The expression profiles analysis showed Ssu-Hsps tissue-specific expression patterns, and they were induced by at least one abiotic stress. The expression correlation between two paralogous pairs (Ssu-sHsp22.2-CV/23.0-CV and 23.8-MT/25.6-MT) were less than 0.6, indicating that they were divergent during the evolution. Various cis-acting elements related to stress responses, hormone or development, were detected in the promoter of Ssu-sHsps. Furthermore, the co-expression network revealed the potential mechanism of Ssu-sHsps under stress tolerance and development. These results provide a foundation for further functional research on the Ssu-sHsp gene family in S. suchowensis.