文献类型：期刊文献

英文题名：Proteomic reconfigurations underlying physiological alterations in poplar roots in acclimation to changing nitrogen availability

作者：Li, Zhuorong[1] Deng, Shurong[1] Zhu, Dongyue[1] Wu, Jiangting[1] Zhou, Jing[1] Shi, Wenguang[1,2] Fayyaz, Payam[3] Luo, Zhi-Bin[1,2] Luo, Jie[2,4]

通信作者：Luo, ZB[1];Luo, J[2]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Natl Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Northwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China;[3]Univ Yasuj, Agr & Nat Resources Fac, Forest Range & Watershed Management Dept, Yasuj 75919 63179, Iran;[4]Huazhong Agr Univ, Coll Hort & Forestry Sci, Hubei Engn Technol Res Ctr Forestry Informat, Wuhan 430070, Peoples R China

年份：2023

卷号：211

外文期刊名：ENVIRONMENTAL AND EXPERIMENTAL BOTANY

收录：;Scopus(收录号：2-s2.0-85157972368);WOS:【SCI-EXPANDED(收录号:WOS:001001681700001)】；

基金：Funding statement This study was jointly supported by the National Key Research and Development Program (Grant no. 2022YFD2201400) and the National Natural Science Foundation of China (Grant Nos. 32171768, 31901287 and 31901282) and the Fundamental Research Funds for the Central Universities (Grant no. 2262022YLYJ007) .

语种：英文

外文关键词：Amino acid; Ammonium transporter; Nitrogen assimilation; Nutrient; Phytohormone; Populus; Proteome

摘要：Although the roots of woody plants are essential for nitrogen (N) uptake and assimilation, proteomic regulation underlying these processes is unclear in acclimation to changing N availability. Here, we carried out physiological and proteomic assays in Populus x canescens sapling roots treated with one of low, normal and high levels of NH4NO3. N deficiency induced lower concentrations of NH4+, total N, amino acids, inhibited enzymatic activities of nitrate reductase and glutamate synthase, decreased levels of abscisic acid and salicylic acid in poplar roots, and high N had the opposite effects. There were 552 and 247 significantly differentially abundant proteins (DAPs) in low and high vs normal N-supplied poplar roots, respectively. Notably, the abundances of a few poplar proteins, including nitrite reductase 1, glutamate synthase 1, and glutamate dehydrogenase 1, corresponded well to N-induced physiological changes in poplar roots. Functional characterization of N-responsive poplar ammonium transporter 2;1 and glutamate dehydrogenase 2 suggests both play pivotal roles in N uptake and assimilation in response to altering N levels. These results suggest that the growth, N uptake and assimilation, and phytohormonal levels are inhibited by N deficiency, but stimulated by high N, and the proteomic reconfigurations occur underlying these physiological alterations in poplars in acclimation to changing N availability.