文献类型：期刊文献

中文题名：韩国栗疫病抗病品种的遗传变异分析

英文题名：Genetic variation of chestnut cultivars for resistance to Cryphonectria parasitica in Korea

作者：朴春根[1] Kim Kyung-hee[2] Lee Sang-hyun[2] Lee Seung-kyu[2]

第一作者：朴春根

机构：[1]中国林业科学研究院森林生态环境与保护研究所国家林业局森林保护学重点实验室;[2]Division of Forest Insect Pests and Diseases,Korea Forest Research Institute,Seoul130-712,Korea

年份：2007

卷号：34

期号：2

起止页码：153-156

中文期刊名：植物保护学报

外文期刊名：Acta Phytophylacica Sinica

收录：CSTPCD;;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：韩国科学财团(KOSEF)Brain Pool Program资助

语种：中文

中文关键词：栗树;栗疫病菌;遗传变异

外文关键词：Castanea spp. ; Cryphonectria parasitica; genetic variation

分类号：S968.313

摘要：为了分析韩国栗疫病的抗病品种和感病品种的遗传变异和抗病性的筛选,利用抗病性的快速检测法和RAPD(random amplified polymorphic DNA)方法对13个栗树品种进行了抗病性检测和RAPD标记分析。抗病性的快速检测选出了5个抗病品种、5个感病品种和3个中度抗病(或中度感病)品种,并且这一结果与该品种的田间表现相一致。利用筛选的12个随机引物,扩增了100个多态性RAPD片段,但未发现与抗病性或感病性相关的特异RAPD片段。聚类分析结果表明,12个品种大致分为抗病、感病和中度抗病(或中度感病)等3个大组,并与抗病性的快速检测结果基本一致。抗病品种“MANSEKI”表现出了相对于12个品种较远的亲缘关系。
Resistance of 13 chestnut cultivars （ Castanea spp. ） to Cryphonectria parasitica in Korea was determined by the rapid testing method. Five chestnut cultivars are resistant, five cultivars susceptible and three cultivars moderate resistant （or susceptible）. The genetic variation was investigated by the random amplified polymorphie DVA（RAPD）, 12 random primers Were selected in RAPD analysis, 100 polymorphic RAPD bands were amplified, but the peculiar RAPD band which discriminates between resistant and susceptible cultivars could not be detected. For the linkage result of cluster analysis, 12 vars could be classified as the resistant, susceptible and moderate resistant （or susceptible） groups approximately, which are almost coordinated to the above rapid testing results. The genetic relationship between one resistant cultivar and other 12 cultivars is very tow. These results suggest that the primers selection of genetic regions related to resistance will be effective for the research of genetic variation and identification of resistance in chestnut.