文献类型：期刊文献

英文题名：Integrated multi-omics analysis reveals flavonoid-driven mechanisms underlying callus browning in Cunninghamia lanceolata

作者：Chen, Huiling[1,2] He, Zhengquan[1] Yang, Yuying[2] Zhao, Yazhi[1,2] Liu, Huan[1,2] Li, Jiayue[2] Li, Minghao[2] Yu, Longhua[3] Feng, Xudong[4] Zhuo, Renying[2] Li, Yueqiao[3] Han, Xiaojiao[2]

第一作者：Chen, Huiling

通信作者：Han, XJ[1];Li, YQ[2]

机构：[1]China Three Gorges Univ, Biotechnol Res Ctr, Key Lab Three Gorges Reg Plant Genet & Germplasm E, Yichang 443002, Hubei, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, State Key Lab Tree Genet & Breeding, Zhejiang Key Lab Forest Genet & Breeding, Hangzhou 311400, Zhejiang, Peoples R China;[3]Chinese Acad Forestry, Expt Ctr Subtrop Forestry, Xinyu 336600, Jiangxi, Peoples R China;[4]Kaihua Cty Forestry Dev, Quzhou 324000, Zhejiang, Peoples R China

年份：2026

卷号：164

期号：2

外文期刊名：PLANT CELL TISSUE AND ORGAN CULTURE

收录：;EI(收录号：20260620018685);Scopus(收录号：2-s2.0-105029075522);WOS:【SCI-EXPANDED(收录号:WOS:001678853900001)】；

基金：This work was funded by The National Key Research and Development Program of China (No. 2023YFD2200104) and Forest and Seedling Cultivation Subsidy Project of the 2025 Provincial Forestry Special Fund for Superior Seed Bases of Cunninghamia lanceolata and Schima superba in Jiangxi Province, China (No. 300100208).

语种：英文

外文关键词：Callus browning; Chinese fir; Multi-omics integrated analysis; Flavonoids; Somatic embryogenesis

摘要：Somatic embryogenesis (SE) represents a canonical manifestation of cell totipotency in plants. However, its application in woody species like Chinese fir (Cunninghamia lanceolata) is limited by callus browning and low embryogenic callus induction frequency. Here, we systematically compared non-embryogenic, embryogenic, and browning callus tissues by the integrated analysis of transcriptomic, proteomic, and targeted metabolomic data in Chinese fir. Immature embryo-derived non-embryogenic callus generated pearl-like embryogenic callus upon subculture, yet browning-induced cellular damage in both types severely impaired somatic embryogenesis. Furthermore, the phenylpropane flavonoid pathway is strongly and coordinately activated in browning callus, with key biosynthetic genes such as PAL, 4CL, and CHS and their encoded proteins consistently up-regulated. Among 183 detected flavonoid metabolites, 23 showed differential accumulation, and 15 were specifically enriched in browning tissue. In addition, we constructed a multi-level evidence chain spanning genes, proteins and metabolites, which revealed that the enzymatic oxidation of phenolic compounds into highly reactive flavonoid derivatives (especially free aglycones), acts as the core mechanism driving callus browning and concomitantly inhibiting embryogenic conversion. The findings not only advance understanding of SE limitations in perennial woody plants but also offer a broadly applicable framework for improving tissue culture systems in other recalcitrant tree species.