文献类型：期刊文献

英文题名：Diverse Plant-Associated Pleosporalean Fungi from Saline Areas: Ecological Tolerance and Nitrogen-Status Dependent Effects on Plant Growth

作者：Qin, Yuan[1] Pan, Xueyu[1] Kubicek, Christian[2] Druzhinina, Irina[2] Chenthamara, Komal[2] Labbe, Jessy[3] Yuan, Zhilin[1]

第一作者：Qin, Yuan

通信作者：Yuan, ZL[1]

机构：[1]Chinese Acad Forestry, Inst Subtrop Forestry, Hangzhou, Zhejiang, Peoples R China;[2]TU Wien, Inst Chem Engn, Res Area Biochem Technol, Vienna, Austria;[3]Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN USA

年份：2017

卷号：8

期号：FEB

外文期刊名：FRONTIERS IN MICROBIOLOGY

收录：;Scopus(收录号：2-s2.0-85014535234);WOS:【SCI-EXPANDED(收录号:WOS:000393333900001)】；

基金：This work was financially supported by the National Natural Science Foundation of China (No. 31370704) and the Fundamental Research Funds for the Central Non-profit Research Institution of RISF-CAF (RISF2013005). JL was supported by the U.S. Department of Energy, Office of Science, Biological and Environmental Research as part of the Plant-Microbe Interfaces Scientific Focus Area (http://pmi.ornl.gov). Oak Ridge National Laboratory is managed by UT-Battelle, LLC, for the U.S. Department of Energy under contract DE-AC05-00OR22725. We would like to express our sincere thanks to Prof. Liyan (Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences) and Prof. Xinhua (School of Life Science, Qingdao Agricultural University) for helping us collect and identify the halophytes. We also greatly appreciate Dr. David Weston (Biosciences Division, Oak Ridge National Laboratory) for improving the language.

语种：英文

外文关键词：Pleosporales; dark septate endophytes; halophytes; organic nitrogen; symbiosis

摘要：Similar to mycorrhizal mutualists, the rhizospheric and endophytic fungi are alsoconsidered to act as active regulators of host fitness (e.g., nutrition and stress tolerance).Despite considerable work in selected model systems, it is generally poorly understoodhow plant-associated fungi are structured in habitats with extreme conditions and towhat extent they contribute to improved plant performance. Here, we investigate thecommunity composition of root and seed-associated fungi from six halophytes growingin saline areas of China, and found that the pleosporalean taxa (Ascomycota) were mostfrequently isolated across samples. A total of twenty-seven representative isolates wereselected for construction of the phylogeny based on the multi-locus data (partial 18SrDNA, 28S rDNA, and transcription elongation factor 1-a), which classified them intoseven families, one clade potentially representing a novel lineage. Fungal isolates weresubjected to growth response assays by imposing temperature, pH, ionic and osmoticconditions. The fungi had a wide pH tolerance, while most isolates showed a variabledegree of sensitivity to increasing concentration of either salt or sorbitol. Subsequentplant-fungal co-culture assays indicated that most isolates had only neutral or evenadverse effects on plant growth in the presence of inorganic nitrogen. Interestingly,when provided with organic nitrogen sources the majority of the isolates enhancedplant growth especially aboveground biomass. Most of the fungi preferred organicnitrogen over its inorganic counterpart, suggesting that these fungi can readily mineralizeorganic nitrogen into inorganic nitrogen. Microscopy revealed that several isolatescan successfully colonize roots and form melanized hyphae and/or microsclerotia-likestructures within cortical cells suggesting a phylogenetic assignment as dark septateendophytes. This work provides a better understanding of the symbiotic relationshipbetween plants and pleosporalean fungi, and initial evidence for the use of this fungalgroup in benefiting plant production.