文献类型：期刊文献

英文题名：Multi-residue detection of pesticides using a sensitive immunochip assay based on nanogold enhancement

作者：Lan, Meijing[1] Guo, Yirong[1] Zhao, Ying[1] Liu, Yihua[1,2] Gui, Wenjun[1] Zhu, Guonian[1]

第一作者：Lan, Meijing

通信作者：Guo, YR[1]

机构：[1]Zhejiang Univ, Inst Pesticide & Environm Toxicol, Hangzhou 310058, Zhejiang, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Fuyang 311400, Peoples R China

年份：2016

卷号：938

起止页码：146-155

外文期刊名：ANALYTICA CHIMICA ACTA

收录：;EI(收录号：20164502998546);Scopus(收录号：2-s2.0-84994201505);WOS:【SCI-EXPANDED(收录号:WOS:000383929700017)】；

基金：This work was financially supported by the Special Fund for Agro-scientific Research in the Public Interest (No. 201203094-3), National Natural Science Foundation of China (No. 31401768), Fundamental Research Fund for the Central Universities (2014QNA6022), and Science-Technology Project Fund in Zhejiang Province of China (2016C32004).

语种：英文

外文关键词：Pesticides; Multi-residue; Microarray; Immunoassay; Gold nanoparticles

摘要：This paper describes the development of a new multiplex immunoassay for simultaneous detection of seven pesticides (triazophos, methyl-parathion, fenpropathrin, carbofuran, thiacloprid, chlorothalonil, and carbendazim). Sixteen pairs of pesticide antibodies and antigens were screened for reactivity and cross-reaction. A microarray chip consisting of seven antigens immobilized on a nitrocellulose membrane was then constructed. Nanogold was employed for labeling and signal amplification to obtain a sensitive colorimetric immunoassay. The direct and indirect detection formats were further compared using primary antibody-gold and secondary antibody-gold conjugates as tracers. An integrated 7-plex immunochip assay based on the indirect model was established and optimized. The detection limits for the pesticides were 0.02-6.45 ng mL(-1), which meets detection requirements for pesticide residues. Naked-eye assessment showed the visual detection limits of the assay ranged from 1 to 100 ng mL(-1). Spiked recovery results demonstrated that the immunochip assay had potential for multi-analysis of pesticide residues in vegetables and fruits. The proposed microarray methodology is a flexible and versatile tool, which can be applied to other competitive multiplex immunoassays for small molecular compounds. (C) 2016 Elsevier B.V. All rights reserved.