文献类型：期刊文献

英文题名：Overexpression of CaAPX Induces Orchestrated Reactive Oxygen Scavenging and Enhances Cold and Heat Tolerances in Tobacco

作者：Wang, Jiangying[1,2] Wu, Bin[1] Yin, Hengfu[1] Fan, Zhengqi[1] Li, Xinlei[1] Ni, Sui[3] He, Libo[1,4] Li, Jiyuan[1]

第一作者：Wang, Jiangying

通信作者：Yin, HF[1];He, LB[1];Li, JY[1];He, LB[2]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Zhejiang Prov Key Lab Tree Breeding, Hangzhou 311400, Zhejiang, Peoples R China;[2]Lianyungang Acad Agr Sci, Flower Res Ctr, Lianyungang 222000, Jiangsu, Peoples R China;[3]Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China;[4]Hunan Agr Univ, Coll Hort & Landscape Architecture, Changsha 410128, Hunan, Peoples R China

年份：2017

卷号：2017

外文期刊名：BIOMED RESEARCH INTERNATIONAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000398788200001)】；

基金：The authors thank Professor Zhongchi Liu from the University of Maryland (College Park) for help with experimental design and technical assistance. This work was supported by funds from Zhejiang Science and Technology Major Program on Agricultural New Variety Breeding (2016C02056-12). They also acknowledge SFA Introduction Program on International Advanced Forestry Sci. & Tech. (2014-4-16), MOST International Sci. & Tech. Cooperation Program (2016YFE0126100), CAF Special Grant on International Cooperation & Innovation (CAFYBB2017ZF001), MOST Nonprofit Sector Special Research Funds on Forestry (201504707), and Ningbo Special Sci. & Tech. Program on Agriculture (2014C11002).

语种：英文

摘要：Ascorbate peroxidase (APX) acts indispensably in synthesizing L-ascorbate (AsA) which is pivotal to plant stress tolerance by detoxifying reactive oxygen species (ROS). Enhanced activity of APX has been shown to be a key step for genetic engineering of improving plant tolerance. However it needs a deeper understanding on the maintenance of cellular ROS homeostasis in response to stress. In this study, we identified and characterized an APX (CaAPX) gene from Camellia azalea. Quantitative real-time PCR (qRT-PCR) analysis showed that CaAPX was expressed in all tissues and peaked in immature green fruits; the expression levels were significantly upregulated upon cold and hot stresses. Transgenic plants displayed marked enhancements of tolerance under both cold and heat treatments, and plant growth was correlated with CaAPX expression levels. Furthermore, we monitored the activities of several ROS-scavenging enzymes including Cu/Zn-SOD, CAT, DHAR, and MDHAR, and we showed that stress tolerance was synchronized with elevated activities of ROS-scavenging. Moreover, gene expression analysis of ROS-scavenging enzymes revealed a role of CaAPX to orchestrate ROS signaling in response to temperature stresses. Overall, this study presents a comprehensive characterization of cellular response related to CaAPX expression and provides insights to breed crops with high temperature tolerances.