文献类型：期刊文献

英文题名：Comamonas endophytica sp. nov., a novel indole acetic acid producing endophyte isolated from bamboo in China

作者：Yue, Jinjun[1] Yang, Fu[2] Xiao, Youtie[3] Lin, Seqiao[3] He, Zhengping[3] Wang, Siyu[2] Zhao, Jiayi[2] Yuan, Jinling[1] Li, Lubin[2] Liu, Lei[2]

第一作者：岳晋军

通信作者：Liu, L[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Fuyang 311400, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, Natl Forestry & Grassland Adm, Key Lab Tree Breeding & Cultivat, Beijing 100091, Peoples R China;[3]Hunan Jiuyishan Natl Nat Reserve Adm Bur, Changsha 425603, Hunan, Peoples R China

年份：2024

卷号：74

期号：1

外文期刊名：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY

收录：;Scopus(收录号：2-s2.0-85181740518);WOS:【SCI-EXPANDED(收录号:WOS:001203460400008)】；

基金：Funding information This research was funded by the Fundamental Research Funds for the Central Non-profit Research Institution of Chinese Academy of Forestry CAFYBB2022XE001.

语种：英文

外文关键词：Comamonas; Indosasa shibataeoides; Phyllostachys edulis; polyphasic approach; phylogeny

摘要：Two novel indole acetic acid- producing strains, 5MLIRT and D4N7, were isolated from Indosasa shibataeoides in Yongzhou, Hunan province, and Phyllostachys edulis in Hangzhou, Zhejiang province, respectively. Based on their 16S rRNA sequences, strains 5MLIRT and D4N7 were closely related to Comamonas antarcticus 16-35-5T (98.4 % sequence similarity), and the results of 92 - core gene phylogenetic trees showed that strains 5MLIRT and D4N7 formed a phylogenetic lineage within the clade comprising Comamonas species. The complete genome size of strain 5MLIRT was 4.49 Mb including two plasmids, and the DNA G+C content was 66.5 mol%. The draft genome of strain D4N7 was 4.26 Mb with 66.7 mol% G+C content. The average nucleotide identity and digital DNA-DNA hybridization values among strain 5MLIRT and species in the genus Comamonas were all below the species delineation threshold. The colonies of strain 5MLIRT and D4N7 were circular with regular margins, convex, pale yellow and 1.0-2.0 mm in diameter when incubated at 30 degrees C for 3 days. Strains 5MLIRT and D4N7 grew optimally at 30 degrees C, pH 7.0 and 1.0 % NaCl. The respiratory isoprenoid quinone was ubiquinone- 8. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Polyphasic analyses indicated that strains 5MLIRT and D4N7 could be distinguished from related validly named Comamonas species and represent a novel species of the genus Comamonas, for which the name Comamonas endophytica sp. nov. is proposed. The type strain is 5MLIRT (=ACCC 62069T=GDMCC 1.2958T=JCM 35331T).