文献类型：期刊文献

中文题名：不同密度杂种落叶松人工林土壤细菌群落对凋落物分解过程的响应

英文题名：Response of Soil Bacteria Community to Leaf Litter Decomposition of Larch(Larix olgensis×Larix kaempferi) Plantation with Different Forest Densities

作者：林英华[1] 汪来发[2] 卢萍[1,2] 谭飞[1,3] 徐演鹏[1,3] 孙楠[4]

第一作者：林英华

机构：[1]中国林业科学研究院湿地研究所;[2]中国林业科学研究院森林生态环境与保护研究所国家林业局森林保护学重点实验室;[3]东北林业大学野生动物资源学院;[4]黑龙江省林业科学院

年份：2013

卷号：49

期号：12

起止页码：101-107

中文期刊名：林业科学

外文期刊名：Scientia Silvae Sinicae

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：国家自然科学基金项目(31071904);国家科技支撑计划项目(2012BAD24B06)

语种：中文

中文关键词：落叶松;细菌群落多样性;凋落物分解袋;动态变化;土壤性质;典型相关分析

外文关键词：larch; soil bacteria community diversity; litterbag; dynamic; soil property; canonicalcorrespondence analysis

分类号：S718.8

摘要：应用稀释平板法和16S RNA序列分析法对不同密度杂种落叶松长白落叶松又日本落叶松人工林2种孔径凋落袋,即上层网孔2 mm、下层网孔1 mm的异孔分解袋和网孔均为1/300 mm的同孔分解袋中凋落物分解过程中土壤细菌群落及多样性进行研究。结果表明:土壤细菌数量因凋落物分解而发生明显变化(F=10.97,P<0.01),其中以芽孢杆菌、假单胞菌、葡萄球菌为代表的纤维素、半纤维素分解菌数量最多。2种孔径凋落袋下菌落的变化不一致:同孔分解袋中菌落随造林密度加大而增多,优势现象明显;异孔分解袋中菌落数随造林密度增加而减少,优势现象不显著。土壤细菌的组成与多样性主要受采样时段影响。不同密度杂种落叶松林土壤细菌群落具有很高的相似性。造林密度对2种凋落袋分解过程中土壤细菌群落的多样性与复杂性影响不一致。典型相关分析表明:土壤细菌群落生存的环境条件较为相似,土壤K对土壤细菌分布影响最大,土壤C/N对土壤细菌分布影响最小。
Soil bacteria community and their diversity were studied during the leaf litter decomposition of Larix olgensis x Larix kaempferi plantation with different densities by two mesh size of litterbags, that is, top layer of 2 mm mesh and bottom layer of 1 mm mesh （mixed mesh litterbag）, as well as 1/300 mm mesh size（ same mesh litterbag）of litterbags. The bacteria were investigated with the plate cultivation method and PCR-16S RNA sequence analysis. The result showed that the number of soil bacteria was changed significantly （ F = 10.97, P 〈 0.01 ） during leaf litter decomposition. The bacteria participating in cellulose, hemicellulose decomposition, such as Bacillus, Pseudomonas, Staphylococcus, was the most dominant. The variety of bacteria community was not consistent between the two types of litterbags. The number of bacteria in the same mesh litterbag increased with the plantation density and some species were obviously dominated, while the number of bacteria in the mixed mesh litterbag decreased with the plantation density and there were no significant dominant species. The composition and diversity of soil bacteria community were mainly influenced by the sampling period. Soil bacteria communities were similar among the different densities of Larix olgensis x Larix kaempferi plantation. The plantation density had different effect on soil bacterial diversity and complexity during leaf litter decomposition of two types of litterbags. The canonical correlation analysis showed that the environmental conditions suitable to soil bacterial community were similar. Soil potassium had the most effect on distribution of soil bacteria, while the ratio of soil carbon tonitrogen had the least effect.