文献类型：期刊文献

英文题名：Plant residues do not have an immediate impact on soil bacterial community composition and abundance

作者：Yuan, Chaolei[1,2,4] Sun, Zhaoyang[1,2] Li, Jing[3]

第一作者：Yuan, Chaolei

通信作者：Yuan, CL[1]

机构：[1]Nankai Univ, Coll Environm Sci & Engn, Tianjin, Peoples R China;[2]Sun Yat Sen Univ, Sch Agr, Shenzhen, Peoples R China;[3]Chinese Acad Forestry, Inst Wetland Res, Beijing Key Lab Wetland Ecol Funct & Restorat, Beijing, Peoples R China;[4]Sun Yet Sen Univ, Sch Agr, 66 Gongchang Rd, Shenzhen 518107, Peoples R China

年份：2023

卷号：74

期号：2

外文期刊名：EUROPEAN JOURNAL OF SOIL SCIENCE

收录：;Scopus(收录号：2-s2.0-85158960974);WOS:【SCI-EXPANDED(收录号:WOS:000970053900001)】；

基金：ACKNOWLEDGEMENTS This work was supported by the National Key Research and Development Program of China (2020YFC1807000), the National Natural Science Foundation of China (41977273, U21A20291), and the Major Research Plan of the Shandong Science Foundation (ZR2020ZD19). Jing Li also acknowledges funding from the Fundamental Research Funds of the Chinese Academy of Forestry (CAFYBB2020QB008) and the Beijing Natural Science Foundation (8214067). We thank Mr. Diansong Yuan and Prof. Zhanhuan Shang for their assistance with soil sampling. We also thank the editors and reviewers for their constructive comments.

语种：英文

外文关键词：interference; plant residue; qPCR; sequencing; soil bacterial community

摘要：Plant residues are often used as soil amendments in laboratory experiments, but they can reportedly release compounds interfering with soil DNA extraction and subsequent molecular biological analyses. Theoretically, for accurate comparison of microbial community composition in soils with and without added plant residues after a period of incubation, no significant difference at the beginning of the experiment is required between the amended and unamended control soils. We mixed plant residue into soil and immediately (within 10 min) commenced DNA extraction, and then performed 16S rRNA gene sequencing and quantitative PCR (qPCR) to determine bacterial community composition and abundance. Soil without plant residue addition served as a control. Five commonly used DNA extraction kits, 16S rRNA gene primer pairs, and soils, and two types (rice straw and alfalfa shoots) and three addition rates (2%, 4%, and 6%; w/w) of plant residue, were tested. In all cases, we found no significant difference in measured bacterial community composition or abundance between the treatments with and without added plant residue.