文献类型：期刊文献

英文题名：High throughput sequencing technology reveals that the taxoid elicitor methyl jasmonate regulates microRNA expression in Chinese yew (Taxus chinensis)

作者：Qiu, Deyou[1] Pan, Xiaoping[2] Wilson, Iain W.[3] Li, Fenglan[1,4,5] Liu, Min[1] Teng, Wenjing[1] Zhang, Baohong[6]

第一作者：邱德有

通信作者：Qiu, DY[1]|[a000598d4c276c5f9aea6]邱德有;

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Dept Mol Biol, Beijing 100091, Peoples R China;[2]Western Illinois Univ, Dept Chem, Macomb, IL 61455 USA;[3]CSIRO Plant Ind, Canberra, ACT 2001, Australia;[4]Chinese Acad Med Sci, Inst Med Plant Dev, Beijing 100094, Peoples R China;[5]Peking Union Med Univ, Beijing 100094, Peoples R China;[6]E Carolina Univ, Dept Biol, Greenville, NC 27858 USA

年份：2009

卷号：436

期号：1-2

起止页码：37-44

外文期刊名：GENE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000265343000006)】；

基金：This work was partially supported by the Natural Science Foundation of China (30671698 and 30440033), National High-Tech Program (2007AA10Z182), Special Research Fund for non-profit Research Institute from The Research Institute of Forestry (2007-01) and East Carolina University New Faculty Research Startup Funds Program (B.Z.). We thank Shujie Wang, Xiaohong Chen, Zhiyingji and Yingchun Hao of CapitalBio for assistance with RNA extraction. We thank Beijing Genomics Institute (BGI) for assistance with sequencing.

语种：英文

外文关键词：MicroRNA; Taxus chinensis; Illumina sequencing; Methyl jasmonate; Paclitaxel biosynthesis

摘要：MicroRNAs (miRNAs) are important regulators of gene expression that are increasing being implicated in controlling plant development and its interaction with the environment. The advent of new high-throughput sequencing technologies has enabled both the discovery and quantification of miRNAs from a diverse range of species. In this study, we employed high throughput Illumina sequencing to identify miRNAs from Taxus chinensis (T chinensis) cells to investigate the effect of the taxoid elicitor methyl jasmonate (MJ) on miRNA expression. In a dataset of similar to 6.6 million sequences, a total of 58 miRNAs, belonging to 25 families were identified. A majority of them are conserved between angiosperms and gymnosperms. However, two miRNAs (miR1310 and miR1314) appear gymnosperm-specific, with miR1314 likely to exist as a cluster. MJ treatment significantly affected the expression of specific miRNAs; 14 miRNAs from 7 different families (miR156, miR168, miR169, miR172, miR396, miR480 and mir1310) were down regulated whereas 3 miRNAs from 2 families (miR164 and miR390) were up regulated. (C) 2009 Elsevier B.V. All rights reserved.