文献类型：期刊文献

中文题名：2株昆虫病原真菌的鉴定及其对松墨天牛幼虫的生物活性测定

英文题名：Identification of Two Entomopathogenic Fungi and Determination of Their Biological Activities Against Monochamus alternatus Hope Larvae

作者：赵纪成[1,2] 王子轩鑫[1] 刘程林[1] 曲良建[1,3] 张龙娃[2] 王青华[1]

第一作者：赵纪成

机构：[1]中国林业科学研究院森林生态环境与自然保护研究所国家林业和草原局森林保护学重点实验室,北京100091;[2]安徽农业大学林学与园林学院,安徽合肥230000;[3]南京林业大学南方现代林业协同创新中心,江苏南京210037

年份：2025

卷号：38

期号：5

起止页码：163-173

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：;北大核心:【北大核心2023】；

基金：中国林业科学研究院基本科研业务费(CAFYBB2021ZG001,CAFYBB2022ZB001)。

语种：中文

中文关键词：松墨天牛;球孢白僵菌;生物学特性;分子鉴定;生物活性

外文关键词：Monochamus alternatus;Beauveria bassiana;Biological characteristics;Molecular identification;Biological activity

分类号：S763.38

摘要：[目的]明确2株昆虫病原真菌的种类及生物学特性,测定它们对松墨天牛(Monochamus alternatus Hope)幼虫的侵染活力。[方法]对2株不同寄主和地区来源的昆虫病原真菌进行分离和纯化,并基于形态学特征及r DNA-ITS、TEF-1α和Bloc序列的同源性明确其分类地位。观察并测定2菌株的分生孢子大小、菌落生长速率、产孢量、孢子萌发率以及对松墨天牛3龄幼虫的生物活性。[结果]形态学观察及多基因序列比对分析结果显示,2株昆虫病原真菌均为球孢白僵菌(Beauveria bassiana),分别编号为LCL-8和MLY-9。其分生孢子均为圆球形或近圆球形,大小分别为(2.414±0.210)μm和(2.681±0.225)μm。生物学特性测定结果表明,LCL-8和MLY-9在PDA培养基(25℃)上的菌落生长速率分别为(4.050±0.052) mm·d^(-1)和(3.825±0.022) mm·d^(-1),产孢量分别为(8.504±0.814)×10^(7)孢子·cm^(-2)和(7.592±0.260)×10^(7)孢子·cm^(-2),24 h孢子萌发率分别为(82.2±4.3)%和(93.2±3.2)%。其中,2菌株在孢子大小、菌落生长速率和24 h孢子萌发率上存在显著性差异(p<0.05)。生测结果显示,LCL-8和MLY-9对松墨天牛3龄幼虫均具有强致病力,且MLY-9的毒力更高。当浓度为1×10^(8)孢子·mL^(-1)时,LCL-8和MLY-9对试虫的累计校正死亡率分别在第11 d和第8 d达到100.0%,其LT50分别为5.470 d和5.335 d。此外,LCL-8和MLY-9对松墨天牛3龄幼虫的LC_(50)分别为5.447×10^(6)孢子·mL^(-1)和8.733×10^(5)孢子·mL^(-1)。[结论]球孢白僵菌LCL-8和MLY-9对松墨天牛幼虫的致病力较高,可作为该害虫绿色防控的潜在生防资源。
[Objective]This study aimed to identify the pathogenic species and biological characteristics of two isolates of entomogenous fungi and evaluate their infection activity against Monochamus alternatus Hope larvae.[Method]Two isolates of entomopathogenic fungi isolated from different hosts and regions were purified and identified using morphological characteristics and sequence homology analysis of rDNA-ITS,TEF-1α,and Bloc regions.The spore diameter,colony growth rate,sporulation,spore germination rate,and biological activity against the 3rd instar larvae of M.alternatus were measured and compared.[Result]Both isolates were identified as Beauveria bassiana,designated as LCL-8 and MLY-9,respect-ively.The conidia of these isolates were spherical or nearly spherical,with diameters of(2.414±0.210)μm and(2.681±0.225)μm,respectively.Colony growth rates on PDA medium at 25℃were(4.050±0.052)mm·d^(-1) and(3.825±0.022)mm·d^(-1),and sporulation rates were(8.504±0.814)×10^(7) spores·cm^(-2) and(7.592±0.260)×10^(7) spores·cm^(-2),respectively.Spore germination rates at 24 h were(82.2±4.3)%for LCL-8 and(93.2±3.2)%for MLY-9.There were significant differences in spore diameters,colony growth rate and 24 h spore germination rate between the two strains(p<0.05).Both isolates showed high patho-genicity against the 3rd instar larvae of M.alternatus,with MLY-9 exhibiting higher virulence.At a concen-tration of 1×10^(8) spores·mL^(-1),the cumulative corrected mortality rates of LCL-8 and MLY-9 reached 100.0%on the 11th and 8th days post-inoculation,respectively,with LT50 values of 5.470 days and 5.335 days.Additionally,the LC_(50) values for LCL-8 and MLY-9 against the larvae were 5.447×10^(6) spores·mL^(-1) and 8.733×105 spores·mL^(-1),respectively.[Conclusion]Beauveira bassiana isolates LCL-8 and MLY-9 exhibit high pathogenicity against Monochamus alternatus larvae,making them promising biocontrol agents for the green prevention and management of this pest.