文献类型：期刊文献

英文题名：A high-density genetic map of Schima superba based on its chromosomal characteristics

作者：Zhang, Rui[1,2] Yang, Hanbo[1,2,3] Zhou, Zhichun[1,2] Shen, Bin[4] Xiao, Jijun[4] Wang, Bangshun[4]

第一作者：Zhang, Rui;张蕊

通信作者：Zhang, R[1];Zhou, ZC[1];Zhang, R[2];Zhou, ZC[2]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Zhejiang, Peoples R China;[2]Zhejiang Prov Key Lab Tree Breeding, Hangzhou 311400, Zhejiang, Peoples R China;[3]Sichuan Acad Forestry, Chengdu 610081, Sichuan, Peoples R China;[4]Longquan Acad Forestry, Longquan 323700, Zhejiang, Peoples R China

年份：2019

卷号：19

期号：1

外文期刊名：BMC PLANT BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000456706800001)】；

基金：The research was financially supported by Zhejiang Provincial Natural Science Foundation of China under Grant No. LQ19C160002, the Tree Breeding Research Project during the 13th Five-year Period of Zhejiang Province (2016C02056-3), the Fifth Stages of Planting and Seedling Science and Technology of Fujian Province (201605), and the Key projects in Jiangxi Province (201503). Authors declare that none of the funding bodies have any role in the design of the study and collection, analysis, and interpretation of data as well as in writing the manuscript.

语种：英文

外文关键词：Theaceae; Schima superba; Chromosome; Karyotype; Genotyping by sequencing (GBS); SNP; Linkage map; Growth traits; QTL

摘要：BackgroundSchima superba (Theaceae) is a popular woody tree in China. The obscure chromosomal characters of this species are a limitation in the development of high-density genetic linkage maps, which are valuable resources for molecular breeding and functional genomics.ResultsWe determined the chromosome number and the karyotype of S. superba as 2n=36=36m, which is consistent with the tribe Schimeae (n=18). A high-density genetic map was constructed using genotyping by sequencing (GBS). A F1 full-sib with 116 individuals and their parents (LC31xJO32) were sequenced on the Illumina HiSeq platform. Overall, 343.3 Gb of raw data containing 1,191,933,474 paired-end reads were generated. Based on this, 99,966 polymorphic SNP markers were developed from the parents, and 2209 markers were mapped onto the integrated genetic linkage map after data filtering and SNP genotyping. The map spanned 2076.24cM and was distributed among 18 linkage groups. The average marker interval was 0.94cM. A total of 168 quantitative trait loci (QTLs) for 14 growth traits were identified.ConclusionsThe chromosome number and karyotype of S. superba was 2n=36=36m and a linkage map with 2209 SNP markers was constructed to identify QTLs for growth traits. Our study provides a basis for molecular-assisted breeding and genomic studies, which will contribute towards the future research and genetic improvement of S. superba.