文献类型：期刊文献

英文题名：Screening of Cucumber Fusarium Wilt Bio-Inhibitor: High Sporulation Trichoderma harzianum Mutant Cultured on Moso Bamboo Medium

作者：Zhang, Ning[1,2] Xu, Hao[1,2] Xie, Jingcong[1,2] Cui, Jie-yu[3] Yang, Jing[1,2] Zhao, Jian[1,2] Tong, Yajuan[1,2] Jiang, Jianchun[1,2]

第一作者：张宁;Zhang, Ning

通信作者：Xie, JC[1];Jiang, JC[1];Xie, JC[2];Jiang, JC[2]

机构：[1]Chinese Acad Forestry, Inst Chem Ind Forest Prod, Natl Engn Lab Biomass Chem Utilizat,Key Lab Bioma, Key Lab Chem Engn Forest Prod,Natl Forestry & Gra, Nanjing, Peoples R China;[2]Nanjing Forestry Univ, Coinnovat Ctr Efficient Proc & Utilizat Forest Re, Nanjing, Peoples R China;[3]Nanjing Forestry Univ, Coll Chem Engn, Nanjing, Peoples R China

年份：2021

卷号：12

外文期刊名：FRONTIERS IN MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000745143800001)】；

基金：Funding This study was financially supported by National Natural Science Foundation of China (Grant No. 31770636), Basic Scientific Research Project of Jiangsu Province Biomass Energy and Material Laboratory (Grant No. JSBEM-S-2017011), and Natural Science Foundation of Jiangsu Province (Grant Nos. BK20180153 and BK20200162).

语种：英文

外文关键词：moso bamboo; Trichoderma harzianum; cucumber fusarium wilt; microwave mutagenesis; mutagenesis

摘要：Cucumber fusarium wilt is a soil-borne disease which causes serious production decrease in cucumber cultivation world widely. Extensive using of chemical pesticides has caused serious environmental pollution and economic losses, therefore, it is particularly urgent to develop efficient, safe and pollution-free biopesticide. In this study, a mutant strain of Trichoderma harzianum cultivated in moso bamboo medium was proved to be an efficient bio-inhibitor of the disease. The mutant strain T. harzianum T334, was obtained by three microwave mutagenesis cycles with an irradiation power of 600 W and irradiation time of 40 s. In contrast to the original strain, the inhibition rate on cucumber fusarium wilt of the strain T334 increased from 63 to 78%. In this work, disk milling pretreatment of moso bamboo has shown significant beneficial effects on both biotransformation and sporulation of T334. Its sporulation reached 3.7 x 10(9) cfu/g in mushroom bags with 90% bamboo stem powder (pretreated by disk milli), 9.5% bamboo leaf powder and 0.5% wheat bran when the ratio of solid to liquid was 4:6, the inoculum amount was 10%, and the culture temperature was 28 degrees C. These results provide an alternative bioinhibitor for the control of cucumber fusarium wilt, and a potential usage of moso bamboo in the production of microbial pesticide.