文献类型：期刊文献

英文题名：Transcriptomic and Metabolomic Analyses Reveal Differences in Flavonoid Pathway Gene Expression Profiles between Two Dendrobium Varieties during Vernalization

作者：Shu, Wenbo[1,2] Shi, Meirong[2] Zhang, Qiqi[1] Xie, Wenyu[1] Chu, Liwei[2] Qiu, Mingxuan[1] Li, Linyan[1] Zeng, Zhixin[1] Han, Lei[2] Sun, Zhenyuan[2]

第一作者：Shu, Wenbo

通信作者：Sun, ZY[1]

机构：[1]Huazhong Agr Univ, Coll Hort & Forestry, Natl Key Lab Germplasm Innovat & Utilizat Crops, Wuhan 430070, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Forestry Adm, Key Lab Tree Breeding & Cultivat, Beijing 100091, Peoples R China

年份：2023

卷号：24

期号：13

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;Scopus(收录号：2-s2.0-85164943380);WOS:【SCI-EXPANDED(收录号:WOS:001028058200001)】；

基金：This work was financially supported by the National Key Research and Development Program of China (2018YFD1000400), Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization (202303102), and the Science and Technology Innovation Fund of the College of Horticulture and Forestry, Huazhong Agricultural University (2023YLSRF14).

语种：英文

外文关键词：Dendrobium capillipes; Dendrobium nobile; vernalization; transcriptome; metabolome; flavonoid biosynthesis; flavone and flavonol biosynthesis

摘要：Dendrobium (Orchidaceae, Epidendoideae) plants have flowers with a wide variety of colors that persist for a long period throughout the year. The yellow coloration of Dendrobium flowers is mainly determined by the flavonol pathway and the flavone pathway, but the relevant biosynthesis mechanisms during vernalization remain unclear. To explore the similarities and differences in flavonoid biosynthesis in different tissues during vernalization, we selected two species of Dendrobium for a flower color study: Dendrobium capillipes Rchb (which has yellow flowers) and Dendrobium nobile Lindl (which has white flowers). We collected a total of 36 samples from six tissue types and both Dendrobium species during vernalization and subjected the samples to metabolic profiling and transcriptome sequencing. A total of 31,504 differentially expressed genes (DEGs) were identified between different tissues of the two Dendrobium species by transcriptomic analysis. However, many differentially accumulated metabolites (DAMs) and DEGs were enriched not only in the general pathway of "flavonoid biosynthesis" but also in multiple subpathways of "flavone and flavonol biosynthesis". According to a combined transcriptome and metabolome analysis, Putrescine hydroxycinnamoyl transferase 1 (LOC110093422) may be the main gene responsible for the differences in flavonoid accumulation during vernalization, which is closely associated with yellow flowers. Taken together, the results of our study preliminarily revealed the metabolites responsible for and the key genes regulating flavonoid biosynthesis during vernalization. These results provide a basis for the further study of the molecular mechanism of flavonoid synthesis during vernalization.