文献类型：期刊文献

英文题名：Populus euphratica remorin 6.5 activates plasma membrane H+-ATPases to mediate salt tolerance

作者：Zhang, Huilong[1,2] Deng, Chen[1] Wu, Xia[1] Yao, Jun[1] Zhang, Yanli[1] Zhang, Yinan[3] Deng, Shurong[4] Zhao, Nan[1] Zhao, Rui[1] Zhou, Xiaoyang[1] Lu, Cunfu[1] Lin, Shanzhi[1] Chen, Shaoliang[1]

第一作者：Zhang, Huilong

通信作者：Lin, SZ[1];Chen, SL[1]

机构：[1]Beijing Forestry Univ, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Coll Biol Sci & Technol, Box 162, Beijing 100083, Peoples R China;[2]Chinese Acad Forestry, Tianjin Res Inst Forestry, Tianjin 300450, Peoples R China;[3]Chinese Acad Forestry, Forestry Inst New Technol, Beijing 100091, Peoples R China;[4]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：2020

卷号：40

期号：6

起止页码：731-745

外文期刊名：TREE PHYSIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000569076700005)】；

基金：The research was supported jointly by the Fundamental Research Funds for the Central Universities (grant no. 2019ZY25), National Natural Science Foundation of China (grant nos. 31770643, 31570587), Natural Science Foundation of Beijing municipality (grant nos. 6182030, 6172024), the Program of Introducing Talents of Discipline to Universities (111 Project, grant no. B13007) and the Beijing Advanced Innovation Center for Tree Breeding by Molecular Design (Beijing Forestry University).

语种：英文

外文关键词：H+-ATPase; H+ flux; NaCl; Na+ flux; plasma membrane vesicles; poplar; remorin; RIN4

摘要：Remorins (REMs) play an important role in the ability of plants to adapt to adverse environments. PeREM6.5, a protein of the REM family in Populus euphratica (salt-resistant poplar), was induced by NaCl stress in callus, roots and leaves. We cloned the full-length PeREM6.5 from P. euphratica and transformed it into Escherichia coli and Arabidopsis thaliana. PeREM6.5 recombinant protein significantly increased the H+-ATPase hydrolytic activity and H+ transport activity in P. euphratica plasma membrane (PM) vesicles. Yeast two-hybrid assay showed that P. euphratica REM6.5 interacted with RPM1-interacting protein 4 (PeRIN4). Notably, the PeREM6.5-induced increase in PM H+-ATPase activity was enhanced by PeRIN4 recombinant protein. Overexpression of PeREM6.5 in Arabidopsis significantly improved salt tolerance in transgenic plants in terms of survival rate, root growth, electrolyte leakage and malondialdehyde content. Arabidopsis plants overexpressing PeREM6.5 retained high PM H+-ATPase activity in both in vivo and in vitro assays. PeREM6.5-transgenic plants had reduced accumulation of Na+ due to the Na+ extrusion promoted by the H+-ATPases. Moreover, the H+ pumps caused hyperpolarization of the PM, which reduced the K+ loss mediated by the depolarization-activated channels in the PM of salinized roots. Therefore, we conclude that PeREM6.5 regulated H+-ATPase activity in the PM, thus enhancing the plant capacity to maintain ionic homeostasis under salinity.