文献类型：期刊文献

英文题名：Involvement of microRNA-Mediated Gene Expression Regulation in the Pathological Development of Stem Canker Disease in Populus trichocarpa

作者：Zhao, Jia-Ping[1,2] Jiang, Xiao-Ling[2,3] Zhang, Bing-Yu[4] Su, Xiao-Hua[4]

第一作者：Zhao, Jia-Ping

通信作者：Zhao, JP[1]

机构：[1]Beijing Forestry Univ, Coll Forestry, Minist Educ, Key Lab Silviculture & Conservat, Beijing, Peoples R China;[2]Chinese Acad Forestry, Inst New Forestry Technol, State Key Lab Forest Genet & Breeding, Beijing, Peoples R China;[3]Agr Univ Hebei, Coll Forestry, Baoding, Peoples R China;[4]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China

年份：2012

卷号：7

期号：9

外文期刊名：PLOS ONE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000311313900059)】；

基金：This work was financially supported by National Program on Key Basic Research Project of China (973 Program) (Grant No. 2009CB119107), National Natural Science Foundation of China (Grant No: 30872048), National Key Technology Research and Development Program of the Ministry of Science and Technology of China during the "12th Five-Year Plan" (Grant No: 2012BAD01B03), and JPZ thanks for China Postdoctoral Science Foundation (Grant No: 20110490302). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

摘要：MicroRNAs (miRNAs), a type of short (21-23 nucleotides), non-coding RNA molecule, mediate repressive gene regulation through RNA silencing at the post-transcriptional level, and play an important role in defense and response to abiotic and biotic stresses. In the present study, Affymetrix (R) miRNA Array, real-time quantitative PCR (qPCR) for miRNAs and their targets, and miRNA promoter analysis were used to validate the gene expression patterns of miRNAs in Populus trichocarpa plantlets induced with the poplar stem canker pathogen, Botryosphaeria dothidea. Twelve miRNAs (miR156, miR159, miR160, miR164, miR166, miR168, miR172, miR319, miR398, miR408, miR1448, and miR1450) were upregulated in the stem bark of P. trichocarpa, but no downregulated miRNAs were found. Based on analysis of the miRNAs and their targets, a potential co-regulatory network was developed to describe post-transcriptional regulation in the pathological development of poplar stem canker. There was highly complex cross-talk between diverse miRNA pathway responses to biotic and abiotic stresses. The results suggest that miR156 is probably an integral component of the miRNA response to all environmental stresses in plants. Cis-regulatory elements were binding sites for the transcription factors (TFs) on DNA. Promoter analysis revealed that TC-rich repeats and a W1-box motif were both tightly related disease response motifs in Populus. Promoter analysis and target analysis of miRNAs also revealed that some TFs regulate their activation/repression. Furthermore, a feedback regulatory network in the pathological development of poplar stem canker is provided. The results confirm that miRNA pathways regulate gene expression during the pathological development of plant disease, and provide new insights into understanding the onset and development of poplar stem canker.