文献类型：期刊文献

英文题名：Transcriptome and DNA methylome provide insights into the molecular regulation of drought stress in sea buckthorn

作者：Lyu, Zhongrui[1] Zhang, Guoyun[1] Song, Yating[1] Diao, Songfeng[3,4] He, Caiyun[1] Zhang, Jianguo[1,2]

第一作者：Lyu, Zhongrui

通信作者：He, CY[1];Zhang, JG[1]|[a0005b245645e47e67b16]何彩云;

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat State Forestry &, Beijing 100091, Peoples R China;[2]Nanjing Forestry Univ, Collaborat Innovat Ctr Sustainable Forestry South, Nanjing 210037, Peoples R China;[3]Chinese Acad Forestry, Nontimber Forestry Res & Dev Ctr, Zhengzhou 450003, Peoples R China;[4]Natl Forestry & Grassland Adm, Key Lab Nontimber Forest Germplasm Enhancement &, Zhengzhou 450003, Peoples R China

年份：2022

卷号：114

期号：3

外文期刊名：GENOMICS

收录：;Scopus(收录号：2-s2.0-85126836211);WOS:【SCI-EXPANDED(收录号:WOS:000821352700005)】；

基金：This work was supported by the Fundamental Research Funds of CAF (CAFYBB2020SZ001-2), National Natural Science Foundation of China (U2003116), and the Fundamental Research Funds of CAF (CAFYBB2020SY006). We are grateful to Guori Gao, Jiayi Li and Hongmei Luo technical assistance.

语种：英文

外文关键词：Sea buckthorn; Drought stress; Methylome; Whole-transcriptome RNA-seq; lncRNAs; circRNAs

摘要：Sea buckthorn is a typical drought-resistant tree species. However, there is a general lack of understanding of the pattern of DNA methylation linked with sea buckthorn responses to drought, and its relationship with drought tolerance mechanisms. In this study, we performed whole-transcriptome RNA sequencing and methylome sequencing in response to drought stress to explore differentially expressed mRNAs, miRNAs, lncRNAs and circRNAs in sea buckthorn leaves. Based on predicted DE pairs, we constructed a competitive endogenous RNA network, which revealed potential transcriptional regulatory roles in response to drought stress. The results of methylome sequencing revealed that the DNA methylation level was increased in sea buckthorn leaves under drought stress. We identified 13,405 differentially methylated regions between CK and TR. We found one DMR-associated DEG (Vacuolar-sorting receptor 6) involved in the ABA accumulation pathway. In addition, two DNA methyltransferases (HrMET1 and HrDRM1) were closely associated with drought-induced hypermethylation in sea buckthorn. Together, we firstly conducted a comprehensive transcriptomic and epigenetic analysis of sea buckthorn under drought stress, providing a resource for further study of the potential functions of genes, miRNAs, lncRNAs, circRNAs and DNA methyltransferases.