文献类型：期刊文献

英文题名：RAPD analysis of genetic variation in natural populations of Betula alnoides from Guangxi, China

作者：Zeng J.[1] Zou Y.[2] Bai J.[1] Zheng H.[1]

第一作者：曾杰

通信作者：Zeng, J[1]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Peoples R China;[2]Chinese Acad Sci, Inst Bot, Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China

年份：2003

卷号：134

期号：1

起止页码：33-41

外文期刊名：EUPHYTICA

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000185962100005)】；

基金：The authors thank Dr Gan S.M., Mr. Chen Z.G., Mr. Weng Q.J. and Mr. Feng C.L. for their field assistance. We are also grateful to Dr Zhang F.M., Dr Ge S. and Dr He T.L. of the Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Science for their assistance in data analysis and suggestions on the manuscript. This study was financially supported by State Forestry Administration, P.R. China (96-011-02-02-03 and 2002BA515B0204).

语种：英文

外文关键词：allozyme; Betula alnoides; genetic diversity; population genetic structure; RAPD

摘要：Random amplified polymorphicDNA (RAPD) markers were used to estimate the genetic variation of seven natural populations of Betula alnoides from Guangxi Zhuang Autonomous Region, China. 16 primers generated a total of 131 highly reproducible and discernible loci, among which 84 were polymorphic. The high genetic diversity was assessed using these RAPD markers at the species and population level, which was congruent with those revealed with allozyme markers, although disparities were found between both types of markers at the single population level. Approximately 90.0% of genetic variance was observed within populations, as revealed with both phenotypic and genotypic RAPD data. The weak differentiation was, perhaps, due to extensive gene flow (Nm > 1.0) of this species in the area. No significant correlation was found between genetic and geographic distances. Based on the results presented in this study, we recommend that a small number of populations represented by large numbers of individuals will be enough for breeding and germplasm conservation purposes, and major attention should be paid to population 2 for conservation and breeding consideration.