文献类型：期刊文献

英文题名：The complete chloroplast genomes of three Betulaceae species: implications for molecular phylogeny and historical biogeography

作者：Yang, Zhen[1] Wang, Guixi[1] Ma, Qinghua[1] Ma, Wenxu[1] Liang, Lisong[1] Zhao, Tiantian[1]

第一作者：杨振

通信作者：Zhao, TT[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat State Forestry &, Beijing, Peoples R China

年份：2019

卷号：7

期号：1

外文期刊名：PEERJ

收录：;Scopus(收录号：2-s2.0-85060650949);WOS:【SCI-EXPANDED(收录号:WOS:000456676500009)】；

基金：This study was supported by the Special Fund for Basic Scientific Research Business of Central Public Research Institutes (Grant Nos. CAFYBB2018SY011 and CAFYBB2017ZA004-9) and the National Natural Science Foundation of China (Grant No. 31500555). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

外文关键词：Betulaceae; Chloroplast genome; Divergence times; Ancestral areas reconstruction; Molecular phylogeny; Comparative genomics

摘要：Background. Previous phylogenetic conclusions on the family Betulaceae were based on either morphological characters or traditional single loci, which may indicate some limitations. The chloroplast genome contains rich polymorphism information, which is very suitable for phylogenetic studies. Thus, we sequenced the chloroplast genome sequences of three Betulaceae species and performed multiple analyses to investigate the genome variation, resolve the phylogenetic relationships, and clarify the divergence history. Methods. Chloroplast genomes were sequenced using the high-throughput sequencing. A comparative genomic analysis was conducted to examine the global genome variation and screen the hotspots. Three chloroplast partitions were used to reconstruct the phylogenetic relationships using Maximum Likelihood and Bayesian Inference approaches. Then, molecular dating and biogeographic inferences were conducted based on the whole chloroplast genome data. Results. Betulaceae chloroplast genomes consisted of a small single-copy region and a large single copy region, and two copies of inverted repeat regions. Nine hotspots can be used as potential DNA barcodes for species delimitation. Phylogenies strongly supported the division of Betulaceae into two subfamilies: Coryloideae and Betuloideae. The phylogenetic position of Ostryopsis davidiana was controversial among different datasets. The divergence time between subfamily Coryloideae and Betuloideae was about 70.49 Mya, and all six extant genera were inferred to have diverged fully by the middle Oligocene. Betulaceae ancestors were probably originated from the ancient Laurasia. Discussions. This research elucidates the potential of chloroplast genome sequences in the application of developing molecular markers, studying evolutionary relationships and historical dynamic of Betulaceae. It also reveals the advantages of using chloroplast genome data to illuminate those phylogenies that have not been well solved yet by traditional approaches in other plants.