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麻竹花药培养及再生植株的获得     被引量:8

Anther Culture and Plant Regeneration of Dendrocalamus latiflorus

文献类型:期刊文献

中文题名:麻竹花药培养及再生植株的获得

英文题名:Anther Culture and Plant Regeneration of Dendrocalamus latiflorus

作者:乔桂荣[1,2] 李海营[1] 蒋晶[1] 孙宗修[2] 卓仁英[1]

第一作者:乔桂荣

机构:[1]中国林业科学研究院亚热带林业研究所;[2]中国水稻研究所

年份:2010

期号:1

起止页码:88-90

中文期刊名:植物学报

外文期刊名:Chinese Bulletin of Botany

收录:CSTPCD;;北大核心:【北大核心2008】;CSCD:【CSCD2011_2012】;

语种:中文

中文关键词:花药培养;麻竹;苯乙酸

外文关键词:anther culture Dendrocalamus latiflorus Munro phenylacetic acid

分类号:S512.103.5;S795.5

摘要:以麻竹(Dendroc alamus latiflorus Munro)花药为材料,于M8+2mg·L-1NAA+0.5mg·L-16-BA+15mg·L-1PAA+7.5mg·L-1STS+500mg·L-1CH+100mg·L-1proline+100mg·L-1glutamin+5.4%maltose+0.8%agar的诱导培养基上成功诱导出胚性愈伤组织,在此培养基上继代可形成体胚并分化成苗,初步建立了麻竹花药一步成苗的再生体系。
Embryogenic callus was initiated from bamboo (Dendrocalamus latiflorus Munro) anthers cultured on M8 medium supplemented with 2 mg·L^–1 NAA, 0.5 mg·L^–1 6-BA, 15 mg·L^–1 phenylacetic acid (PAA), 7.5 mg·L^–1 silver thiosulfate (STS), 500 mg·L^–1 casein enzymatic hydrolysate (CH), 100 mg·L^–1 proline, 100 mg·L^–1 glutamine, 5.4% maltose, and 0.8% agar. Subculture of these embryogenic calli on the same medium resulted in embryoid formation and their subsequent germination to form rooted plantlets. A one-step method for anther culture and plant regeneration of D. latiflorus was preliminarily established.

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