文献类型：期刊文献

英文题名：Molecular cloning and yeast two-hybrid provide new evidence for unique sporophytic self-incompatibility system of Corylus

作者：Hou, S.[1,2,3,4] Zhao, T.[1,2,3,4] Yang, Z.[1,2,3,4] Yang, D.[1,2,3,4] Li, Q.[1,2,3,4] Liang, L.[1,2,3,4] Wang, G.[1,2,3,4] Ma, Q.[1,2,3,4]

第一作者：Hou, S.

通信作者：Ma, Q[1]

机构：[1]State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[2]Chinese Acad Forestry, Key Lab Tree Breeding & Cultivat State Forestry &, Beijing, Peoples R China;[3]Hazelnut Engn & Tech Res Ctr State Forestry & Gra, Beijing, Peoples R China;[4]Natl Forestry & Grassland Innovat Alliance Hazeln, Beijing, Peoples R China

年份：2022

卷号：24

期号：1

起止页码：104-116

外文期刊名：PLANT BIOLOGY

收录：;Scopus(收录号：2-s2.0-85118303068);WOS:【SCI-EXPANDED(收录号:WOS:000713326100001)】；

基金：This work was supported by the Special Fund for Basic Scientific Research Business of Central Public Research Institutes of the Chinese Academy of Forestry, China (CAFYBB2017ZA004-9) and the Special Investigation on Basic Resources of Science and Technology (Grant No. 2019FY100801-03).

语种：英文

外文关键词：Ping'ou hybrid hazelnut (C; heterophylla Fisch; x C; avellana L; ); pistil-pollen interactions; quantitative real-time PCR (qRT-PCR); RACE cloning; yeast two-hybrid

摘要：The Corylus genus contains several important nut producing species and exhibits sporophytic self-incompatibility (SSI). However, the underlying molecular mechanisms of SSI in Corylus remain largely unknown. To clarify whether Corylus and Brassica share the same SSI molecular mechanism. We cloned ChaTHL1/2, ChaMLPK, ChaARC1, ChaEX70A1 genes from Ping'ou hybrid hazelnut using RACE techniques and tested the interaction between the ChaARC1 and ChaSRK1/2. We also examined the pistil-pollen interactions using scanning electron microscopy. We found no differences in the stigma surface within 1 h after compatible or incompatible pollination. Compatible pollen tubes penetrated the stigma surface, while incompatible pollen did not penetrate the stigma 4 h after pollination. Bioinformatics analysis revealed that ChaTHL1/2, ChaMLPK, ChaARC1 and ChaEX70A1 have corresponding functional domains. Quantitative real-time PCR (qRT-PCR) analysis showed that ChaTHL1/2, ChaMLPK, ChaARC1 and ChaEX70A1 were not regularly expressed in compatible or incompatible pollination. Furthermore, the expression patterns of ARC1, THL1/2, MLPK and Exo70A1 were quite distinct between Corylus and Brassica. According to yeast two-hybrid assays, ChaSRK1/2 did not interact with ChaARC1, confirming that the SRK-ARC1 signalling pathway implicated in the SSI response of Brassica was not conserved in Corylus. These results further reinforce the conclusion that, notwithstanding the similarity of the genetic basis, the SSI mechanism of Corylus does not conform in many respects with that of Brassica. Our findings could be helpful to better explore the potential mechanism of SSI system in Corylus.