文献类型：期刊文献

英文题名：Genomewide analysis of the lateral organ boundaries domain gene family in Eucalyptus grandis reveals members that differentially impact secondary growth

作者：Lu, Qiang[1] Shao, Fenjuan[1] Macmillan, Colleen[2] Wilson, Iain W.[2] van der Merwe, Karen[3] Hussey, Steven G.[3] Myburg, Alexander A.[3] Dong, Xiaomei[4,5] Qiu, Deyou[1]

通信作者：Qiu, DY[1]|[a000598d4c276c5f9aea6]邱德有;

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Res Inst Forestry, Beijing, Peoples R China;[2]CSIRO Agr & Food, Canberra, ACT, Australia;[3]Univ Pretoria, FABI, Dept Genet, GRI, Pretoria, South Africa;[4]China Agr Univ, State Key Lab Agrobiotechnol, Dept Plant Genet & Breeding, Beijing, Peoples R China;[5]China Agr Univ, Natl Maize Improvement Ctr, Dept Plant Genet & Breeding, Beijing, Peoples R China

年份：2018

卷号：16

期号：1

起止页码：124-136

外文期刊名：PLANT BIOTECHNOLOGY JOURNAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000423363300012)】；

基金：This work was supported by the Basic Research Fund of RIF (RIF2014-01), Natural Science Foundation of China (31670676), Mondi and Sappi through the Forest Molecular Genetics Programme, the Technology and Human Resources for Industry Programme (UID 80118) and the National Research Foundation (UID 18312, 71255 and 86936) of South Africa. The authors declare no conflict of interest.

语种：英文

外文关键词：Eucalyptus grandis; lateral organ boundaries domain; gene expression; secondary growth; xylem development; fibre production

摘要：Lateral Organ Boundaries Domain (LBD) proteins are plant-specific transcription factors playing crucial roles in growth and development. However, the function of LBD proteins in Eucalyptus grandis remains largely unexplored. In this study, LBD genes in E.grandis were identified and characterized using bioinformatics approaches. Gene expression patterns in various tissues and the transcriptional responses of EgLBDs to exogenous hormones were determined by qRT-PCR. Functions of the selected EgLBDs were studied by ectopically overexpressing in a hybrid poplar (Populus albaxPopulus glandulosa). Expression levels of genes in the transgenic plants were investigated by RNA-seq. Our results showed that there were forty-six EgLBD members in the E.grandis genome and three EgLBDs displayed xylem-(EgLBD29) or phloem-preferential expression (EgLBD22 and EgLBD37). Confocal microscopy indicated that EgLBD22, EgLBD29 and EgLBD37 were localized to the nucleus. Furthermore, we found that EgLBD22, EgLBD29 and EgLBD37 were responsive to the treatments of indol-3-acetic acid and gibberellic acid. More importantly, we demonstrated EgLBDs exerted different influences on secondary growth. Namely, 35S::EgLBD37 led to significantly increased secondary xylem, 35S::EgLBD29 led to greatly increased phloem fibre production, and 35S::EgLBD22 showed no obvious effects. We revealed that key genes related to gibberellin, ethylene and auxin signalling pathway as well as cell expansion were significantly up- or down-regulated in transgenic plants. Our new findings suggest that LBD genes in E.grandis play important roles in secondary growth. This provides new mechanisms to increase wood or fibre production.