文献类型：期刊文献

英文题名：Genome-wide analyses of calmodulin and calmodulin-like proteins in the halophyte Nitraria sibirica reveal their involvement in response to salinity, drought and cold stress

作者：Wu, Xinru[1,2] Zhu, Junjie[1,2] Zhu, Liming[1,2] Tang, Yao[1,2] Hao, Zhaodong[1,2] Zhang, Jingbo[3] Shi, Jisen[1,2] Cheng, Tielong[4] Lu, Lu[1,2]

第一作者：Wu, Xinru

通信作者：Lu, L[1]

机构：[1]Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, State Key Lab Tree Genet & Breeding, Nanjing 210037, Peoples R China;[2]Nanjing Forestry Univ, Key Lab Forest Genet & Biotechnol, Minist Educ, Nanjing 210037, Peoples R China;[3]Chinese Acad Forestry, Expt Ctr Desert Forestry, Dengkou, Inner Mongolia, Peoples R China;[4]Nanjing Forestry Univ, Coll Biol & Environm, Nanjing 210037, Peoples R China

年份：2023

卷号：253

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;EI(收录号：20234214921261);Scopus(收录号：2-s2.0-85174356794);WOS:【SCI-EXPANDED(收录号:WOS:001101189000001)】；

基金：Funding This work was supported by the National Natural Science Foundation of China (31770715) ; and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) .

语种：英文

外文关键词：Halophyte; Calmodulin; Calmodulin-like protein

摘要：The calmodulin (CaM) and calmodulin-like (CML) proteins are major calcium sensors that play a critical role in environmental stimulus response in plants. Nevertheless, the CaM/CML proteins from the specific plants with extreme tolerance to abiotic stresses remained so far uncharacterized. In this study, 66 candidate proteins (three NsCaMs and sixty-three NsCMLs) were identified from the halophyte Nitraria sibirica, which can withstand an extreme salinity. Bioinformatic analysis of upstream cis-acting elements predicted the potential involvement of NsCaM/CMLs in abiotic stress responses and various hormone responses. Additionally, the Nitraria sibirica transcriptome revealed that 17 and 7 NsCMLs were significantly upregulated under 100 mM or 400 mM NaCl treatment. Transcription of most salt-responsive genes was similarly upregulated under cold stress, yet downregulated under drought treatment. Moreover, predictive subcellular localization analysis suggested that the stress-responsive NsCML proteins mainly localize at the cellular membrane and within the nucleus. Furthermore, transgenic overexpression of two NsCMLs (NISI03G1136 and NISI01G1645) was found to mitigate H2O2 accumulation caused by salt stress. These results provide insights into the potential function of Nitraria sibirica CaM/ CML proteins, which could aid the investigation of molecular mechanisms of extreme tolerance to abiotic stresses in halophytes.