文献类型：期刊文献

英文题名：Diverse non-mycorrhizal fungal endophytes inhabiting an epiphytic, medicinal orchid (Dendrobium nobile): estimation and characterization

作者：Yuan, Zhi-lin[1,2] Chen, Yi-cun[1] Yang, Yun[3]

第一作者：Yuan, Zhi-lin;袁志林

通信作者：Yuan, ZL[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Fuyang, Peoples R China;[2]Zhejiang Univ, Inst Biotechnol, Hangzhou 310003, Zhejiang, Peoples R China;[3]Nabanhe Natl Nat Reserve, Xishuangbanna, Yunan Province, Peoples R China

年份：2009

卷号：25

期号：2

起止页码：295-303

外文期刊名：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000263676600015)】；

语种：英文

外文关键词：Fungal endophytes; Dendrobium nobile; Xylaria spp.; Guignardia mangiferae; Clonostachys rosea; Trichoderma chlorosporum; Phylogenetic analysis

摘要：Although the terrestrial and temperate orchids-fungal biology have been largely explored, knowledge of tropical epiphytic orchids-fungus relationships, especially on the ecological roles imparted by non-mycorrhizal fungal endophytes, is less known. Exploitation of the endophytic fungal mycobiota residing in epiphytic orchid plants may be of great importance to further elucidate the fungal ecology in this special habitat as well as developing new approaches for orchid conversations. The composition of fungal endophytes associated with leaves, stems and roots of an epiphytic orchid (Dendrobium nobile), a famous Chinese traditional medicinal plant, was investigated. Microscopic imaging, culture-dependant method and molecular phylogeny were used to estimate their entity and diversity. Totally, there were 172 isolates, at least 14 fungal genera and 33 different morphospecies recovered from 288 samples. Ascomycetes, coelomycetes and hyphomycetes were three major fungal groups. There were higher overall colonization and isolation rates of endophytic fungi from leaves than from other tissues. Guignardia mangiferae was the dominant fungal species within leaves; while the endophytic Xylariaceae were frequently observed in all plant tissues; Colletotrichum, Phomopsis and Fusarium were also frequently observed. Phylogenetic analysis based on ITS gene revealed the high diversity of Xylariacea fungi and relatively diverse of non-Xylariacea fungi. Some potentially promising beneficial fungi such as Clonostachys rosea and Trichoderma chlorosporum were found in roots. This is the first report concerning above-ground and below-ground endophytic fungi community of an epiphytic medicinal orchid, suggesting the ubiquitous distribution of non-mycorrhizal fungal endophytes in orchid plants together with heterogeneity and tissue specificity of the endophyte assemblage. Possible physiological functions played by these fungal endophytes and their potential applications are also discussed briefly.